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Applied Optics

Applied Optics


  • Vol. 24, Iss. 2 — Jan. 15, 1985
  • pp: 194–200

Reconstructing 3-D light-microscopic images by digital image processing

Angelika Erhardt, G. Zinser, D. Komitowski, and J. Bille  »View Author Affiliations

Applied Optics, Vol. 24, Issue 2, pp. 194-200 (1985)

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A reconstruction procedure based on linear system theory has been developed for 3-D light-microscopic images. Inverse filtering with the 3-D optical transfer function was used for image reconstruction. The procedure allows a significant improvement in spatial resolution in the image planes perpendicular to the optical axis.

© 1985 Optical Society of America

Original Manuscript: June 29, 1984
Published: January 15, 1985

Angelika Erhardt, G. Zinser, D. Komitowski, and J. Bille, "Reconstructing 3-D light-microscopic images by digital image processing," Appl. Opt. 24, 194-200 (1985)

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