A reconstruction procedure based on linear system theory has been developed for 3-D light-microscopic images. Inverse filtering with the 3-D optical transfer function was used for image reconstruction. The procedure allows a significant improvement in spatial resolution in the image planes perpendicular to the optical axis.
© 1985 Optical Society of America
Original Manuscript: June 29, 1984
Published: January 15, 1985
Angelika Erhardt, G. Zinser, D. Komitowski, and J. Bille, "Reconstructing 3-D light-microscopic images by digital image processing," Appl. Opt. 24, 194-200 (1985)