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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 24, Iss. 9 — May. 1, 1985
  • pp: 1355–1365

Flow-cytometric light scattering measurement of red blood cell volume and hemoglobin concentration

D. H. Tycko, M. H. Metz, E. A. Epstein, and A. Grinbaum  »View Author Affiliations


Applied Optics, Vol. 24, Issue 9, pp. 1355-1365 (1985)
http://dx.doi.org/10.1364/AO.24.001355


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Abstract

A light scattering technique for simultaneously determining the volume (V) and hemoglobin concentration (HC) of individual sphered red blood cells (RBCs) is described. Light scattered into two angular intervals yields measurements S1 and S2, respectively. Since a sphered RBC is essentially a homogeneous dielectric sphere having a complex refractive index that is linear in HC, with a proper choice of detector acceptance angles, tables relating V and HC to S1 and S2 can be computed via Mie theory. Absolute calibration is possible using droplets of water-immisible oils of accurately known refractive index. Results of experimental tests of the method are compared with those obtained from hematological reference measurements.

© 1985 Optical Society of America

History
Original Manuscript: October 19, 1984
Published: May 1, 1985

Citation
D. H. Tycko, M. H. Metz, E. A. Epstein, and A. Grinbaum, "Flow-cytometric light scattering measurement of red blood cell volume and hemoglobin concentration," Appl. Opt. 24, 1355-1365 (1985)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-24-9-1355

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