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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 25, Iss. 7 — Apr. 1, 1986
  • pp: 1215–1221

Absolute calibration of a fluorescence collection system by Raman scattering in H2

William K. Bischel, Douglas J. Bamford, and Leonard E. Jusinski  »View Author Affiliations


Applied Optics, Vol. 25, Issue 7, pp. 1215-1221 (1986)
http://dx.doi.org/10.1364/AO.25.001215


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Abstract

A new method for absolute calibration of the detection efficiency of fluorescence collection systems is described for wavelengths covering the 180–2000-nm range. The radiation source for the technique is spontaneous Raman scattering in H2. An analytic expression is derived for the scattering cross section averaged over the solid angle of the fluorescence collection system. From this result, an optical system is calibrated at 845 nm, demonstrating that the technique is easy to use, is quick to implement, and gives accurate results.

© 1986 Optical Society of America

Citation
William K. Bischel, Douglas J. Bamford, and Leonard E. Jusinski, "Absolute calibration of a fluorescence collection system by Raman scattering in H2," Appl. Opt. 25, 1215-1221 (1986)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-25-7-1215

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