Abstract

The light microscope coupled to appropriate video equipment and image-processing computers now yields images with hitherto unobtainable clarity and resolution. Objects far smaller than the limit of resolution can be visualized to reveal their dynamic behavior in several contrast modes. This paper reviews the role of video contrast enhancement which makes possible the optimal use of these hidden, inherent capabilities of the light microscope. Analog and digital video image enhancement as well as intensifier video cameras are used to bring out microscopic and submicroscopic features of living cells and dynamic molecular assemblies unsuspected before the application of video microscopy.

© 1987 Optical Society of America

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