OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 26, Iss. 16 — Aug. 15, 1987
  • pp: 3226–3231

Laser scanning fluorescence microscopy

Johan S. Ploem  »View Author Affiliations


Applied Optics, Vol. 26, Issue 16, pp. 3226-3231 (1987)
http://dx.doi.org/10.1364/AO.26.003226


View Full Text Article

Enhanced HTML    Acrobat PDF (1948 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Fluorescence laser scanning microscopy (LSM) offers many advantages over conventional fluorescence microscopy. Very strong excitation light can be concentrated on small spots (0.5 μm) of the specimen, enabling the detection of low concentrations of fluorescent substances. The low levels of autofluorescence generated in the microscope objective and in the immersion oil in LSM provide images of great contrast, even with weakly fluorescent specimens. Confocal LSM permits the visualization of multiple focal layers of the specimen and 3-D image reconstructions. Combination of images stored in computer memory allow the comparison of phase contrast and fluorescence images of the same area of the specimen enabling multiparameter analysis of cells.

© 1987 Optical Society of America

History
Original Manuscript: May 13, 1987
Published: August 15, 1987

Citation
Johan S. Ploem, "Laser scanning fluorescence microscopy," Appl. Opt. 26, 3226-3231 (1987)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-26-16-3226

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

If you are accessing the full text through a member bundle, please use the Enhanced HTML link to gain access to the citation lists and other restricted features. Note that accessing both the PDF and HTML versions of an article will count as only one download against your account.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

If you are accessing the full text through a member bundle, please use the Enhanced HTML link to gain access to the citation lists and other restricted features. Note that accessing both the PDF and HTML versions of an article will count as only one download against your account.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

If you wish to use one of your free member downloads to view the figures, click "Enhanced HTML" above and access the figures from the article itself or from the navigation tab.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

If you are accessing the full text through a member bundle, please use the Enhanced HTML link to gain access to the citation lists and other restricted features. Note that accessing both the PDF and HTML versions of an article will count as only one download against your account.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited