Abstract

The AMAC IIIS electrooptical transducer for flow cytometry facilitates simultaneous measurements of electrical impedance and several optical properties of single cells. Ray-trace analysis demonstrates that its spherical exterior permits light rays to emerge effectively undeviated and thus lowers the required numerical aperture of the collecting lens as compared with a flat surfaced transducer. This feature results in an increased light-gathering ability, an increased depth of focus, minimum spherical and chromatic aberrations, and minimizes the optical volume observed.

© 1987 Optical Society of America

Ray tracing analysis of the image quality of a high collection efficiency mirror system

Newton K. Seitzinger, John C. Martin, and Richard A. Keller
Appl. Opt. 29(28) 4255-4258 (1990)

Two-color method for optical astrometry: theory and preliminary measurements with the Mark III stellar interferometer

M. Mark Colavita, Michael Shao, and David H. Staelin
Appl. Opt. 26(19) 4113-4122 (1987)

Accuracy of optical density measurement of cells. 1: Low resolution

A. W. M. Smeulders and T. K. ten Kate
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Image DNA-index (ploidy) analysis in cancer diagnosis

P. S. Oud, A. G. J. M. Hanselaar, M. M. M. Pahlplatz, J. W. R. Meijer, and G. Peter Vooijs
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Automated single-cell manipulation and sorting by light trapping

Tudor N. Buican, Miriam J. Smyth, Harry A. Crissman, Gary C. Salzman, Carleton C. Stewart, and John C. Martin
Appl. Opt. 26(24) 5311-5316 (1987)