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Applied Optics

Applied Optics


  • Vol. 28, Iss. 6 — Mar. 15, 1989
  • pp: 1061–1077

Spectral reflectance of the human ocular fundus

François C. Delori and Kent P. Pflibsen  »View Author Affiliations

Applied Optics, Vol. 28, Issue 6, pp. 1061-1077 (1989)

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Reflectance spectra from discrete sites in the human ocular fundus were measured with an experimental reflectometer in the visible and near-infrared parts of the spectrum. The principal study population consisted of ten subjects 22 to 38 years of age withba wide range of degree of fundus melanin pigmentation. Reflectance spectra were obtained from the nasal fundus, the fovea, and an area 2.5° from the fovea. Spectra were also recorded from several older subjects and from one aphakic patient with a coloboma. The reflectance spectra were found to be influenced by the degree of individual and local melanin pigmentation of the fundus, the amount of blood in the choroid, the transmission properties of the ocular media, and the discrete reflections in the stratified fundus layers. Mathematical models of the optical properties of the stratified layers are proposed and are fitted to the experimental fundus reflectance spectra. The models account for the absorption by blood, melanin, macular pigment, and ocular media, and incorporate tissue scattering and discrete reflectors corresponding to anatomical layers.

© 1989 Optical Society of America

Original Manuscript: July 5, 1988
Published: March 15, 1989

François C. Delori and Kent P. Pflibsen, "Spectral reflectance of the human ocular fundus," Appl. Opt. 28, 1061-1077 (1989)

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  1. J. J. Vos, A. A. Munnik, J. Boogaard, “Absolute Spectral Reflectance of the Fundus Oculi,” J. Opt. Soc. Am. 55, 573 (1965). [CrossRef]
  2. R. A. Weale, “Polarized Light and the Human Fundus Oculi,” J. Physiol. 186, 175 (1966). [PubMed]
  3. R. Rohler, U. Miller, M. Aberl, “Zur Messung der Modulationsiibertragungsfunktion des Lebenden Menschlichen Auges im Reflektierten Licht,” Vision Res. 9, 407 (1969). [CrossRef] [PubMed]
  4. M. Millodot, “Reflection from the Fundus of the Eye and Its Relevance to Retinoscopy,” Atti Fond. Giorgio Ronchi 27, 31 (1972).
  5. W. N. Charman, J. A. M. Jennings, “Objective Measurements of the Longitudinal Chromatic Aberration of the Human Eye,” Vision Res. 16, 999 (1976). [CrossRef] [PubMed]
  6. R. W. Flower, D. S. McLeod, S. M. Pitts, “Reflection of Light by Small Areas of the Ocular Fundus,” Invest. Ophthalmol. Vis. Sci. 16, 981 (1977). [PubMed]
  7. D. O’Leary, M. Millodot, “The Discrepancy Between Retinoscopic and Subjective Refraction: Effect of Light Polarization,” Am. J. Optom. Physiol. Opt. 55, 553 (1978). [CrossRef]
  8. W. N. Charman, “Reflection of Plane-Polarized Light by the Retina,” Br. J. Physiol. Opt. 34, 34 (1980). [PubMed]
  9. J.-M. Gorrand, R. Alfieri, J.-Y. Boire, “Diffusion of the Retinal Layers of the Living Human Eye,” Vision Res. 24, 1097 (1984). [CrossRef] [PubMed]
  10. D. van Norren, L. F. Tiemeijer, “Spectral Reflectance of the Human Eye,” Vision Res. 26, 313 (1986). [CrossRef] [PubMed]
  11. D. van Norren, J. van der Kraats, “A Continuously Recording Retinal Densitometer,” Vision Res. 21, 897 (1981). [CrossRef] [PubMed]
  12. D. J. Faulkner, C. M. Kemp, “Human Rhodopsin Measurement Using a T.V.-Based Imaging Fundus Reflectometer,” Vision Res. 24, 221 (1984). [CrossRef] [PubMed]
  13. P. E. Kilbride, J. S. Read, G. A. Fishman, M. Fishman, “Determination of Human Cone Pigment Density Difference Spectra in Spatially Resolved Regions of the Fovea,” Vision Res. 12, 1341 (1983). [CrossRef]
  14. S. Trokel, “Quantitative Studies of Choroidal Blood Flow by Reflective Densitometry,” Invest. Ophthalmol. 4, 1129 (1965). [PubMed]
  15. J. Gloster, “Fundus Oximetry,” Exp. Eye Res. 6, 187 (1967). [CrossRef] [PubMed]
  16. N. H. Bakker, “Fundus Reflectometry, an Experimental Study,” Doc. Ophthalmol. 38, 271 (1974). [PubMed]
  17. J. Gloster, “Fundus Reflectometry in the Study of the Choroidal Circulation,” Int. Ophthalmol. 6, 109 (1983). [CrossRef] [PubMed]
  18. F. Delori, “Noninvasive Technique for Oximetry of Blood in Retinal Vessels,” Appl. Opt. 27, 1113 (1988). [CrossRef] [PubMed]
  19. K. P. Pflibsen, F. C. Delori, O. Pomerantzeff, M. M. Pankratov, “Fundus Reflectometry for Photocoagulation Dosimetry,” Appl. Opt. 28, 1084 (1989). [CrossRef] [PubMed]
  20. W. Hunold, P. Malessa, “Spectrophotometric Determination of Melanin Pigmentation of the Human Ocular Fundus in vivo,” Ophthalmic Res. 6, 355 (1974). [CrossRef]
  21. G. S. Brindley, E. N. Willmer, “The Reflection of Light from the Macular and Peripheral Fundus Oculi in Man,” J. Physiol. (London) 116, 350 (1952).
  22. K. R. Alexander, P. E. Kilbride, G. A. Fishman, M. Fishman, “Macular Pigment and Reduced Foveal Short-Wavelength Sensitivity in Retinitis Pigmentosa,” Vision Res. 27, 1077 (1987). [CrossRef] [PubMed]
  23. T. Behrendt, T. D. Duane, “Investigation of Fundus Oculi with Spectral Reflectance Photography. I: Depth and Integrity of Fundal Structures,” Arch. Ophthalmol. 75, 375 (1966). [CrossRef] [PubMed]
  24. F. C. Delori, E. S. Gragoudas, R. Francisco, R. C. Pruett, “Monochromatic Ophthalmoscopy and Fundus Photography,” Arch. Ophthalmol. 95, 861 (1977). [CrossRef] [PubMed]
  25. American National Standards Institute, “Safe Use of Lasers,” Z136.1 (ANSI, New York, 1976).
  26. F. Delori, J. S. Parker, M. A. Mainster, “Light Levels in Fundus Photography and Fluorescein Angiography,” Vision Res. 20, 1099 (1980). [CrossRef] [PubMed]
  27. D. van Norren, J. J. Vos, “Spectral Transmission of the Human Ocular Media,” Vision Res. 14, 1237 (1974). [CrossRef]
  28. F. C. Delori, K. P. Plibsen, “Reflectance Properties of the Optic Disc,” Noninvasive Assessment of the Visual System. OSA Technical Digest, in press.
  29. P. R. Bevington, Data Reduction and Error Analysis for the Physical Sciences (McGraw-Hill, New York, 1969), p. 235.
  30. Determination of pigment concentrations requires a tight fit both in red light (melanin) and in green light (hemoglobin). Because of the large differences in reflectance in those two spectral bands (Fig. 2), such fit could be achieved by minimizing the relative errors or by using a weight Wλ=Robs, λ−2 in Eq. (2). However, a lesser weight might be desired for short wavelengths to minimize the effect of inaccuracies caused by ocular media light scattering, low signal-to-noise ratio, etc. We therefore selected Wλ=Robs,λ−1 as a compromise.
  31. J. Neter, W. Wasserman, M. H. Kutner, Applied Linear Statistical Models (Richard D. Irwin, Inc., Homewood, IL, 1985), p. 580.
  32. W. A. Rushton, G. H. Henri, “Bleaching and Regeneration of Cone Pigments in Man,” Vision Res. 8, 617 (1968). [CrossRef] [PubMed]
  33. V. P. Gabel, R. Birngruber, F. Hillekamp, “Visible and Near Infrared Light Absorption in Pigment Epithelium and Choroid,” in Twenty-Third Concilium Ophthalmologicum, Kyoto, K. Shimizu, Ed. (Excerpta Medica, Amsterdam, 1978).
  34. I. A. Menon, S. Persad, H. F. Haberman, C. J. Kurian, P. K. Basu, “A Qualitative Study of the Melanins from Blue and Brown Human Eyes,” Exp. Eye Res. 34, 531 (1982). [CrossRef] [PubMed]
  35. W. J. Geeraets, R. C. Williams, G. Chan, W. T. Ham, D. Guerry, F. H. Schmidt, “The Relative Absorption of Thermal Energy in Retina and Choroid,” Invest. Ophthalmol. 1, 340 (1962). [PubMed]
  36. J. J. Weiter, F. C. Delori, G. L. Wing, K. A. Fitch, “Retinal Pigment Epithelium Lipofuscin and Melanin and Choroidal Melanin in Human Eyes,” Invest. Ophthalmol. Vis. Sci. 27, 145 (1986). [PubMed]
  37. The in vitro measurements of Weiter et al.36 were made with polychromatic light in the 500–600-nm spectral range. Using the melanin absorption spectrum of Fig. 4 (solid curve), we calculated that the absorption coefficient at 500 nm should be about 1.6 times higher than those reported in Weiter’s study.
  38. W. Lerche, “Die Kapillarisierung der Menschlichen Retina,” in Eye Structure, II Symposium, J. W. Rohen, Ed. (Schattauer-Verlag, Stuttgart, 1965).
  39. O. W. van Assendelft, Spectroscopy of Hemoglobin Derivatives (C. C. Thomas, Springfield, IL, 1970).
  40. D. J. Coleman, F. L. Lizzi, “In vivo Choroidal Thickness Measurement,” Am. J. Ophthalmol. 88, 369 (1979). [PubMed]
  41. S. Tane, J. Kohno, J. Horikoshi, K. Kondo, K. Ohashi, A. Komatsu, T. Kakehashi, “The Study on the Microscopic Biometry of the Thickness of the Human Retina, Choroid and Sclera by Ultrasound,” Acta Soc. Ophthalmol. Jpn. 88, 1412 (1984).
  42. M. J. Hogan, J. A. Alvarado, J. E. Weddell, Histology of the Human Eye (Saunders, Philadelphia, 1971).
  43. A. Bill, G. Sperber, K. Ujiie, “Physiology of the Choroidal Vascular Bed,” Int. Ophthalmol. 6, 101 (1983). [CrossRef] [PubMed]
  44. D. M. Snodderly, P. K. Brown, F. C. Delori, J. D. Auran, “The Macular Pigment. I. Absorbance Spectra, Localization, and Discrimination from Other Yellow Pigments in Primate Retinas,” Invest. Ophthalmol. Vis. Sci. 25, 660 (1984). [PubMed]
  45. E. A. Boetner, J. R. Wolter, “Transmission of the Ocular Media,” Invest. Ophthalmol. 1, 776 (1962).
  46. J. Walraven, “Spatial Characteristics of Chromatic Induction: the Segregation of Lateral Effects from Straylight Artefacts,” Vision Res. 13, 1739 (1973). [CrossRef] [PubMed]
  47. R. S. Smith, M. N. Stein, “Ocular Hazards of Transscleral Laser Radiation. I. Spectral Reflection and Transmission of the Sclera, Choroid and Retina,” Am. J. Ophthalmol. 66, 21 (1968). [PubMed]
  48. M. Alpern, S. Thompson, M. S. Lee, “Spectral Transmittance of Visible Light by the Living Human Eye,” J. Opt. Soc. Am. 55, 723 (1965). [CrossRef] [PubMed]
  49. M. T. Mori, R. C. Zeimer, M. F. Goldberg, “Noninvasive Measurement of Retinal Thickness: a Potential Diagnostic Tool for Macular Edema and Atrophy,” Invest. Ophthalmol. Vis. Sci. 29 (ARVO Suppl), 339 (1988).
  50. R. C. Zeimer, U. Illinois, Research Eye Institute; personal communication (June1988).
  51. G. Kortum, Reflectance Spectroscopy (Springer-Verlag, New York, 1969), p. 116.
  52. R. L. Longini, R. Zdrojkowski, “A Note on the Theory of Backscattering of Light by Living Tissue,” IEEE Trans. Biomed. Eng. BME-15, 4 (1968). [CrossRef]
  53. R. R. Anderson, J. A. Parrish, “The Optics of Human Skin,” J. Invest. Dermatol. 77, 13 (1981). [CrossRef] [PubMed]
  54. S. Takatani, M. D. Graham, “Theoretical Analysis of Diffuse Reflectance from a Two-Layer Tissue Model,” IEEE Trans. Biomed. Eng. BME-26, 656 (1979). [CrossRef]
  55. R. A. Bone, J. M. B. Sparrock, “Comparison of Macular Pigment Densities in Human Eyes,” Vision Res. 11, 1057 (1971). [CrossRef] [PubMed]
  56. D. W. Lubbers, R. Wodick, “Absolute Reflection Photometry Applied to the Measurement of Capillary Oxyhaemoglobin Saturation of the Skin in Man,” Oxygen Meas. Biol. Med. 1, 85 (1974).
  57. L. N. M. Duysens, “The Flattening of the Absorption Spectrum of Suspensions, as Compared to that of Solutions,” Biochim. Biophys. Acta 19, 1 (1956). [CrossRef] [PubMed]

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