The resolution of a fluorescent confocal scanning optical microscope (CSOM) is superior to that of a conventional fluorescent optical microscope. To attain this superiority, the fluorescent CSOM uses a pinhole in front of the detector. Thus, the resolution of the CSOM is dependent on the pinhole radius. Three-dimensional optical transfer functions are calculated for the various radii to elucidate this dependence. The results show that a CSOM with a radius smaller than ~1 optical unit has a bandwidth comparable with that of an infinitely small radius.
© 1990 Optical Society of America
Original Manuscript: January 17, 1989
Published: July 10, 1990
Shigeharu Kimura and Chusuke Munakata, "Dependence of 3-D optical transfer functions on the pinhole radius in a fluorescent confocal optical microscope," Appl. Opt. 29, 3007-3011 (1990)