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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 29, Iss. 26 — Sep. 10, 1990
  • pp: 3810–3815

Three-dimensional phase contrast imaging by an annular illumination microscope

Tomoya Noda, Satoshi Kawata, and Shigeo Minami  »View Author Affiliations


Applied Optics, Vol. 29, Issue 26, pp. 3810-3815 (1990)
http://dx.doi.org/10.1364/AO.29.003810


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Abstract

A method of observing 3-D phase structures through a microscope incorporating computer reconstruction is discussed. This microscope is equipped with an annular pupil in illumination optics, but no phase shifter is included in the imaging optics. The sample stage is longitudinally (z-axial) scanned to collect a focus image series. The 3-D phase transfer function is derived and computer-plotted based on Streibl’s 3-D image transfer theory under the first-order Born approximation and the mutual intensity propagation theorem. Experimental results of 3-D phase reconstruction are shown with cultured tobacco cells by Helstrom’s inverse filtering of the transfer function to a series of focused images.

© 1990 Optical Society of America

History
Original Manuscript: January 3, 1990
Published: September 10, 1990

Citation
Tomoya Noda, Satoshi Kawata, and Shigeo Minami, "Three-dimensional phase contrast imaging by an annular illumination microscope," Appl. Opt. 29, 3810-3815 (1990)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-29-26-3810

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