Miniature laser light scattering instrumentation for particle size
analysis

Robert G. W. Brown; James G. Burnett; John Mansbridge; Christopher I. Moir

doi:10.1364/AO.29.004159

Applied Optics
Vol. 29,
Issue 28,
pp. 4159-4169
(1990)
•https://doi.org/10.1364/AO.29.004159

Miniature laser light scattering instrumentation for particle size analysis

Robert G. W. Brown, James G. Burnett, John Mansbridge, and Christopher I. Moir

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Robert G. W. Brown, James G. Burnett, John Mansbridge, and Christopher I. Moir, "Miniature laser light scattering instrumentation for particle size analysis," Appl. Opt. 29, 4159-4169 (1990)

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Abstract

We describe the design, construction, and testing of a miniature, all-solid state laser light scattering instrument for determination of particle sizes and distributions using photon correlation spectroscopy techniques (i.e., quasielastic or dynamic light scattering). Detailed comparative tests with standard photon correlation spectroscopy equipment are presented.

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		Standard PCS	Miniature PCS
Mean diameter, nma		86.08 ± 0.6 (0.22)c
Polydispersity	[40 mW]b	0.064 ± 0.015	86.22 ± 0.59 (0.19)
Polydispersity	[7 mW]	0.041 ± 0.015	0.031 ± 0.023 [5 mW]

		Standard PCS	Miniature PCS
Mean diameter, nm		220.5 ± 3.6 (1.3)b
Polydispersity	[40 mW]a	0.105 ± 0.044	223.7 ± 3.6 (1.3)
Polydispersity	[7 mW]	0.040 ± 0.026	0.026 ± 0.018 [5 mW]

		Standard PCS	Miniature PCS
Mean diameter, nma		530.4 + 17.2 (4.5)c
Polydispersitya	[40 mW]b	0.200 ± 0.066	514.5 ± 24.58 (3.8)
Polydispersitya	[7 mW]	0.187 ± 0.109	0.086 ± 0.05 [5 mW]

Material	Mol. Wt./1000a		Miniature PCS diameter (nm)	Standard PCS diameter (nm)
Thyroglobulin	669	10	23.8 ± 0.1	23.8 ± 0.1
Thyroglobulin	669	2	24.3 ± 0.1	23.8 ± 0.1
Aldolaseb	158	5	13.9 ± 0.3	13.8 ± 0.2
Aldolaseb	158	1	12.5 ± 0.9	13.8 ± 0.2
Ovalbumin	43	10	9.9 ± 0.9	10.4 ± 0.4
Ovalbumin	43	2	9.6 ± 1.1	10.4 ± 0.4
Chymotrypsinogen A	25	20	5.3 ± 0.4	5.4 ± 0.2
Chymotrypsinogen A	25	4	(—)c	5.4 ± 0.2
Lysozyme	14.7	20	5.7 ± 0.7	4.9 ± 0.3
Lysozyme	14.7	14	5.4 ± 0.6)	4.9 ± 0.3
Ribonuclease A	13.7	20	5.8 ± 1.0	5.9 ± 0.49
Ribonuclease A	13.7	4	(—)c	5.9 ± 0.49

		Standard PCS	Miniature PCS
Mean diameter, nma		86.08 ± 0.6 (0.22)c
Polydispersity	[40 mW]b	0.064 ± 0.015	86.22 ± 0.59 (0.19)
Polydispersity	[7 mW]	0.041 ± 0.015	0.031 ± 0.023 [5 mW]

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Applied Optics