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Applied Optics

Applied Optics


  • Vol. 29, Iss. 34 — Dec. 1, 1990
  • pp: 5163–5169

Microscopic mapping of subnanometric motion

Hungyi Lin and Mark Sharnoff  »View Author Affiliations

Applied Optics, Vol. 29, Issue 34, pp. 5163-5169 (1990)

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The sensitivity of a microscope-based holographic system designed for mapping the motion of components of a living cell is calibrated by means of a simple procedure. Bubbles of air are allowed to drift with known velocity in a nearly horizontal, glycerine-filled capillary observed through the microscope. The ultrafine motion of the bubbles is captured interferometrically by subtractive superposition of a pair of holograms recorded a few milliseconds apart. Stationary portions of the field of view are rendered dimly in images reconstructed from the interferogram, while the brightness of moving portions varies monotonically with their displacement. Displacements as small as 1.1 nm were betrayed in the images. Analysis of the experimental conditions suggests that bubble displacements as small as 0.7 nm will become detectable once minor improvements are made in the apparatus.

© 1990 Optical Society of America

Original Manuscript: December 4, 1989
Published: December 1, 1990

Hungyi Lin and Mark Sharnoff, "Microscopic mapping of subnanometric motion," Appl. Opt. 29, 5163-5169 (1990)

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  1. S. Tolansky, Microstructure of Surfaces Using Interferometry (Elsevier, New York, 1978).
  2. D. Gabor, “Holography, 1948–1971,” Science 177, 299–313 (1972). [CrossRef] [PubMed]
  3. A. E. Ennos, “Speckle Interferometry,” in Laser Speckle and Related Phenomena, J. C. Dainty, Ed. (Springer-Verlag, Berlin, 1984), pp. 203–253.
  4. K. A. Stetson, “The Use of an Image Derotator in Hologram Interferometry and Speckle Photography of Rotating Objects,” Exp. Mech. 18, 67–73 (1978). [CrossRef]
  5. S. Inoue, Video Microscopy (Plenum, New York, 1986).
  6. M. Sharnoff, “Microdifferential Holography,” J. Opt. Soc. Am. A 2, 1619–1628 (1985). [CrossRef] [PubMed]
  7. M. Sharnoff, T. H. Karcher, L. P. Brehm, “Microdifferential Holography and the Polysarcomeric Unit of Activation of Skeletal Muscle,” Science 223, 822–825 (1984). [CrossRef] [PubMed]
  8. Bath wax, catalog no. 15-532, Fisher Scientific Co.
  9. H. Gardner, P. Holdt, Paint and Varnish Manufacturing Association of the U.S.A., Circular No. 128 (1921).
  10. R. Hubbard, G. Brown, “The Rolling Ball Viscometer,” Ind. Eng. Chem. 15, 212–218 (1943).
  11. S. Hu, R. C. Kintner, “The Fall of Single Liquid Drops Through Water,” AIChE J. 1, 42–48 (1955). [CrossRef]
  12. A. I. Johnson, L. Braida, “The Velocity of Fall of Circulating and Oscillating Liquid Drops Through Quiescent Liquid Phases,” Can. J. Chem. Eng. 35, 165–172 (1957).
  13. A. Einstein, “Über die von der molekularkinetischen Theorie der Wärme geforderte Bewegung von in ruhenden Flüssigkeiten suspendierten Teilchen,” Ann”. Phys. 17, 549–560 (1905).
  14. E. Leith, J. Upatnieks, “Reconstructed Wavefronts and Communication Theory,” J. Opt. Soc. Am. 52, 1123–1130 (1962). [CrossRef]
  15. R. L. Powell, K. A. Stetson, “Interferometric Vibration Analysis by Wavefront Reconstruction,” J. Opt. Soc. Am. 55, 1593–1598 (1965). [CrossRef]
  16. L. O. Heflinger, R. F. Wuerker, R. E. Brooks, “Holographic Interferometry,” J. Appl. Phys. 37, 642–649 (1966). [CrossRef]
  17. K. A. Haines, B. P. Hildebrand, “Surface-Deformation Measurement Using the Wavefront Reconstruction Technique,” Appl. Opt. 5, 595–602 (1966). [CrossRef] [PubMed]
  18. G. W. Ellis, “Holomicrography: Transformation of Image During Reconstruction a Posteriori,” Science 154, 1195–1196 (1966). [CrossRef] [PubMed]
  19. R. F. Van Ligten, H. Osterberg, “Holographic Microscope,” Nature London 211, 282–283 (1966).
  20. D. Gabor, G. W. Stroke, R. Restrick, A. Funkhouser, D. Brumm, “Optical Image Synthesis (Complex Amplitude Addition and Subtraction) by Holographic Fourier Transformation,” Phys. Lett. 18, 116–118 (1965). [CrossRef]
  21. Type I 1002, Gaertner Instrument Co., Chicago, IL.

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