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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 29, Iss. 4 — Feb. 1, 1990
  • pp: 489–494

Depth resolution of the fluorescent confocal scanning optical microscope

Shigeharu Kimura and Chusuke Munakata  »View Author Affiliations


Applied Optics, Vol. 29, Issue 4, pp. 489-494 (1990)
http://dx.doi.org/10.1364/AO.29.000489


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Abstract

The fluorescent confocal scanning optical microscope is capable of 3-D observation, for which depth discrimination is vital. To characterize the depth discrimination capability of this microscope, intensity variations depending on fluorescence wavelength are simulated for fluorescent films being moved in the depth direction. Also, the resolution between two films is calculated for a range of fluorescence wavelengths. Results of experiments using photoresist films are compared to simulations. As a practical application, through-holes opened in a thick photoresist film are observed by slice images in the depth direction.

© 1990 Optical Society of America

History
Original Manuscript: October 25, 1988
Published: February 1, 1990

Citation
Shigeharu Kimura and Chusuke Munakata, "Depth resolution of the fluorescent confocal scanning optical microscope," Appl. Opt. 29, 489-494 (1990)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-29-4-489


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References

  1. T. Wilson, C. Sheppard, Theory and Practice of Scanning Microscopy (Academic, London, 1984).
  2. C. J. R. Sheppard, T. Wilson, “Depth of Field in the Scanning Microscope,” Opt. Lett. 3, 115–117 (1978). [CrossRef] [PubMed]
  3. C. J. R. Sheppard, “The Spatial Frequency Cut-Off in Three-Dimensional Imaging,” Optik 72, 131–133 (1986).
  4. C. J. R. Sheppard, “The Spatial Frequency Cut-Off in Three-Dimensional Imaging II,” Optik 74, 128–129 (1986).
  5. D. K. Hamilton, T. Wilson, “Surface Profile Measurement Using the Confocal Microscope,” J. Appl. Phys. 53, 5320–5322 (1982). [CrossRef]
  6. D. K. Hamilton, T. Wilson, “Three-Dimensional Surface Measurement Using the Confocal Scanning Microscope,” Appl. Phys. B 27, 211–213 (1982). [CrossRef]
  7. I. J. Cox, C. J. R. Sheppard, T. Wilson, “Super-Resolution by Confocal Fluorescent Microscopy,” Optik 60, 391–396 (1982).
  8. I. J. Cox, “Scanning Optical Fluorescence Microscopy,” J. Microsc. 133, 149–154 (1984). [CrossRef] [PubMed]
  9. T. Wilson, “Optical Sectioning in Confocal Fluorescent Microscopes,” J. Microsc. 154, 143–156 (1989). [CrossRef]
  10. K. Carlsson, N. Åslund, “Confocal Imaging for 3-D Digital Microscopy,” Appl. Opt. 26, 3232–3238 (1987). [CrossRef] [PubMed]
  11. R. W. Wijnaends van Resandt, H. J. B. Marsman, R. Kaplan, J. Davoust, E. H. K. Stelzer, R. Stricker, “Optical Fluorescence Microscopy in Three Dimensions: Microtomoscopy,” J. Microsc. 138, 29–34 (1985). [CrossRef]
  12. W. B. Amos, J. G. White, M. Fordham, “Use of Confocal Imaging in the Study of Biological Structures,” Appl. Opt. 26, 3239–3243 (1987). [CrossRef] [PubMed]
  13. G. J. Brakenhoff, H. T. M. van der Voort, E. A. van Spronsen, N. Nanninga, “3-Dimensional Imaging of Biological Structures by High Resolution Confocal Scanning Laser Microscopy,” Scanning Micros. 2, 33–40 (1988).
  14. H. T. M. van der Voort, G. J. Brakenhoff, G. C. A. M. Janssen, “Determination of the 3-Dimensional Optical Properties of a Confocal Scanning Laser Microscope,” Optik 78, 48–53 (1988).
  15. H. H. Hopkins, “The Frequency Response of a Defocused Optical System,” Proc. R. Soc. London Ser. A 231, 91–103 (1955). [CrossRef]
  16. P. A. Stokseth, “Properties of a Defocused Optical System,” J. Opt. Soc. Am. 59, 1314–1321 (1969). [CrossRef]
  17. T. Wilson, A. R. Carlini, “Depth Discrimination Criteria in Confocal Optical Systems,” Optik 76, 164–166 (1987).
  18. F. A. Jenkins, H. E. White, Fundamentals of Optics (McGraw-Hill, Tokyo, 1981), p. 38.

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