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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 30, Iss. 25 — Sep. 1, 1991
  • pp: 3563–3568

Aberration compensation in confocal microscopy

C. J. R. Sheppard and Min Gu  »View Author Affiliations


Applied Optics, Vol. 30, Issue 25, pp. 3563-3568 (1991)
http://dx.doi.org/10.1364/AO.30.003563


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Abstract

In confocal microscopy, spherical aberration is introduced when one is focusing deep within the specimen. This can be compensated for by altering the effective tube length at which the objective is operated. The limitations of this approach are investigated.

© 1991 Optical Society of America

History
Original Manuscript: December 5, 1990
Published: September 1, 1991

Citation
C. J. R. Sheppard and Min Gu, "Aberration compensation in confocal microscopy," Appl. Opt. 30, 3563-3568 (1991)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-30-25-3563

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