In confocal microscopy, spherical aberration is introduced when one is focusing deep within the specimen. This can be compensated for by altering the effective tube length at which the objective is operated. The limitations of this approach are investigated.
© 1991 Optical Society of America
Original Manuscript: December 5, 1990
Published: September 1, 1991
C. J. R. Sheppard and Min Gu, "Aberration compensation in confocal microscopy," Appl. Opt. 30, 3563-3568 (1991)