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Applied Optics

Applied Optics


  • Vol. 31, Iss. 29 — Oct. 10, 1992
  • pp: 6247–6254

Computed tomography from optical projections for three-dimensional reconstruction of thick objects

Carl S. Brown, David H. Burns, Francis A. Spelman, and Alan C. Nelson  »View Author Affiliations

Applied Optics, Vol. 31, Issue 29, pp. 6247-6254 (1992)

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An optical tomography system is developed for generating three-dimensional reconstructions of thick objects from projections. The system is useful for studying transparent structures that are 1–10 mm in diameter. Evaluation of the reconstruction system with a test object demonstrates 98% geometric accuracy, 90% accuracy in the detection of boundaries, and 90% accuracy in the measurement of absorbance. Reconstructions are computed from 96 parallel projections spaced evenly within 180°. Accurate alignment of the projections is achieved with a cross-correlation method following data acquisition. Application of the optical tomography reconstruction technique to an intact cochlea permits measurement of internal structures with 16-μm pixels and a diffraction-limited resolution of 24 μm.

© 1992 Optical Society of America

Original Manuscript: August 9, 1991
Published: October 10, 1992

Carl S. Brown, David H. Burns, Francis A. Spelman, and Alan C. Nelson, "Computed tomography from optical projections for three-dimensional reconstruction of thick objects," Appl. Opt. 31, 6247-6254 (1992)

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