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Applied Optics

Applied Optics


  • Vol. 31, Iss. 30 — Oct. 20, 1992
  • pp: 6376–6382

Absorption effects on total-internal-reflection fluorescence spectroscopy

Minoru Toriumi, Masatoshi Yanagimachi, and Hiroshi Masuhara  »View Author Affiliations

Applied Optics, Vol. 31, Issue 30, pp. 6376-6382 (1992)

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A theoretical consideration is made for transverse-electric polarization vectors of a transmitted field in an optically absorbing sample to derive a fluorescence intensity formula as a function of the absorption index of the sample as well as incident and observation angles in total-internal-reflection fluorescence spectroscopy. These equations are experimentally confirmed. It is shown that the angular spectrum of fluorescence intensity is the Laplace transformation of the effective penetration depth, even for the absorbing sample. High resolution of the depth profile is expected for an absorbing sample compared with a nonabsorbing one.

© 1992 Optical Society of America

Original Manuscript: June 20, 1991
Published: October 20, 1992

Minoru Toriumi, Masatoshi Yanagimachi, and Hiroshi Masuhara, "Absorption effects on total-internal-reflection fluorescence spectroscopy," Appl. Opt. 31, 6376-6382 (1992)

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  1. N. J. Harrick, Internal Reflection Spectroscopy (Harrick Scientific, New York, 1967).
  2. E. D. Palik, R. T. Holm, “Internal-reflection-spectroscopy studies of thin films and surfaces,” Opt. Eng. 17, 512–524 (1978).
  3. C. K. Carniglia, L. Mandel, K. H. Drexhage, “Absorption and emission of evanescent photons,” J. Opt. Soc. Am. 62, 479–486 (1972). [CrossRef]
  4. C. Allain, D. Ausserre, F. Rondelez, “Direct optical observation of interfacial depletion layers in polymer solutions,” Phys. Rev. Lett. 49, 1694–1697 (1982). [CrossRef]
  5. E. Lee, R. E. Benner, J. B. Fenn, R. K. Chang, “Angular distribution of fluorescence from liquids and monodispersed spheres by evanescent wave excitations,” Appl. Opt. 18, 862–868 (1979). [CrossRef]
  6. M. Toriumi, M. Yanagimachi, H. Masuhara, “Dynamics in the surface layer of the polymer films,” in Dynamics in Small Confining Systems, J.Drake Drake, J.Klafter Klafter, R. Kopelman, eds. (Materials Research Society, Pittsburgh, Pa., 1990), pp. 133–136.
  7. M. Yanagimachi, M. Toriumi, M. Masuhara, “Selective incorporation and aggregation of pyrene in a segmented polyurethaneurea film as revealed by picosecond total internal reflection fluorescence spectroscopy,” Chem. Mater. 3, 413–418 (1991). [CrossRef]
  8. G. Muller, K. Abraham, M. Schaldach, “Quantitative ATR spectroscopy: some basic considerations,” Appl. Opt. 20, 1182–1190 (1981). [CrossRef] [PubMed]
  9. M. Born, E. Wolf, Principles of Optics (Pergamon, Oxford, 1970).
  10. A. Itaya, T. Yamada, K. Tokuda, H. Masuhara, “Interfacial characteristics of poly (methyl methacrylate) film: aggregation of pyrene and micropolarity revealed by time-resolved total internal reflection fluorescence spectroscopy,” Polymer J. 22, 697–704 (1990). [CrossRef]
  11. M. Toriumi, M. Yanagimachi, H. Masuhara, “Structure of poly (p-hydroxystyrene) film,” in Advances in Resist Technology and Processing VIII, H. Ito, ed., Proc. Soc. Photo-Opt. Instrum. Eng.1466, 458–468 (1991).
  12. J. B. Birks, Photophysics of Aromatic Molecules (Wiley-Interscience, London, 1970).
  13. K. Tomioka, M. Iemura, A. Matsui, “Reflectance spectra and optical constatns of pyrene crystals,” J. Phys. Soc. Jpn. 50, 2078–2083 (1981). [CrossRef]

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