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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 33, Iss. 10 — Apr. 1, 1994
  • pp: 1838–1843

Three-dimensional image sensing by chromatic confocal microscopy

H. J. Tiziani and H.-M. Uhde  »View Author Affiliations


Applied Optics, Vol. 33, Issue 10, pp. 1838-1843 (1994)
http://dx.doi.org/10.1364/AO.33.001838


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Abstract

In the image of a confocal microscope, only those parts of an object appear bright that are located in the focal plane of the objective. Because of an axial chromatic aberration deliberately introduced into the microscope objective, the location of the focal plane depends on the wavelength used. By using a white-light source and examining an object with a depth variation less than the axial range of the chromatic focus, we find that all parts of the object appear sharp and bright in the image, but according to its height they appear in different colors. A camera with black-and-white film sequentially combines, with three selected chromatic filters, intensity and tone of color of each object point. For each tone of color one can assign a height by using a calibration curve. This assignment could be made unequivocal by the selection of filters with adequate chromatic transmission.

© 1994 Optical Society of America

History
Original Manuscript: October 8, 1992
Published: April 1, 1994

Citation
H. J. Tiziani and H.-M. Uhde, "Three-dimensional image sensing by chromatic confocal microscopy," Appl. Opt. 33, 1838-1843 (1994)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-33-10-1838

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