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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 33, Iss. 19 — Jul. 1, 1994
  • pp: 4286–4292

Hyper-Rayleigh light scattering from an aqueous suspension of purple membrane

P. K. Schmidt and G. W. Rayfield  »View Author Affiliations


Applied Optics, Vol. 33, Issue 19, pp. 4286-4292 (1994)
http://dx.doi.org/10.1364/AO.33.004286


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Abstract

Here we report the first observation of hyper-Rayleigh light scattering from bacteriorhodopsin in the form of an aqueous suspension of unoriented purple membranes. A typical purple membrane suspension used in our experiments contains approximately 108 randomly oriented purple membranes. Each purple membrane contains approximately 105 bacteriorhodopsin molecules in a two-dimensional crystalline array. Hyper-Rayleigh light scattering is observed when the purple membrane suspension is illuminated with light that has a wavelength of 1064 nm. We propose that the 532-nm scattered light from each of the bacteriorhodopsin molecules in a single purple membrane is coherent, and that the scattered light from different purple membranes is incoherent. This proposal is supported by the following experimental observations: (a) the 532-nm light intensity is proportional to the square of the incident power, (b) the intensity of the 532-nm signal is linearly proportional to the concentration of purple membrane in solution, (c) the scattered 532-nm light is incoherent, (d) the scattered 532-nm light intensity decreases if the size of the purple membranes is reduced while the bacteriorhodopsin concentration is kept constant, and (e) the 532-nm light is due to the retinal chromophore of the bacteriorhodopsin molecule. The ratio of horizontal polarized hyper-Rayleigh scattered light to vertically polarized hyper-Rayleigh scattered light gives the angle (23 ± 4°) of the retinal axis with respect to the plane of the purple membrane. The hyperpolarizability of the bacteriorhodopsin molecule is found to be 5 ± 0.4 × 10−27 esu.

© 1994 Optical Society of America

History
Original Manuscript: June 14, 1993
Revised Manuscript: November 8, 1993
Published: July 1, 1994

Citation
P. K. Schmidt and G. W. Rayfield, "Hyper-Rayleigh light scattering from an aqueous suspension of purple membrane," Appl. Opt. 33, 4286-4292 (1994)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-33-19-4286

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