OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 33, Iss. 22 — Aug. 1, 1994
  • pp: 5204–5213

Quantitative determination of the absorption spectra of chromophores in strongly scattering media: a light-emitting-diode based technique

Sergio Fantini, Maria Angela Franceschini, Joshua B. Fishkin, Beniamino Barbieri, and Enrico Gratton  »View Author Affiliations


Applied Optics, Vol. 33, Issue 22, pp. 5204-5213 (1994)
http://dx.doi.org/10.1364/AO.33.005204


View Full Text Article

Enhanced HTML    Acrobat PDF (1177 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

The absorption and scattering coefficient of a macroscopically homogeneous strongly scattering medium (lipid emulsion) containing Methylene Blue is quantitatively measured in the spectral range from 620 to 700 nm. We conduct the measurements in the frequency domain by using a light-emitting diode (LED) whose intensity is modulated at a frequency of 60 MHz. We derive an analytical expression for the absorption and scattering coefficients that is based on a two-distance measurement technique. A comparison with other measurement protocols such as measurement at two modulation frequencies shows that the two-distance method gives a better determination of the scattering and absorption coefficients. This study highlights the efficiency and ease of use of the LED technique, which lends itself to in vivo spectroscopy of biological tissues.

© 1994 Optical Society of America

History
Original Manuscript: July 6, 1993
Revised Manuscript: January 3, 1994
Published: August 1, 1994

Citation
Sergio Fantini, Maria Angela Franceschini, Joshua B. Fishkin, Beniamino Barbieri, and Enrico Gratton, "Quantitative determination of the absorption spectra of chromophores in strongly scattering media: a light-emitting-diode based technique," Appl. Opt. 33, 5204-5213 (1994)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-33-22-5204


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. G. Wagnieres, C. Depeursinge, P. Monnier, M. Savary, P. Cornaz, A. Chatelain, H. van den Bergh, “Photodetection of early cancer by laser induced fluorescence of a tumor-selective dye: apparatus design and realization,” in Photodynamic Therapy: Mechanisms II, T. J. Dougherty, ed., Proc. Soc. Photo-Opt. Instrum. Eng.1203, 43–52 (1990).
  2. M. S. Patterson, B. C. Wilson, J. W. Feather, D. M. Burns, W. Pushka, “The measurement of dihematopotphyrin ether concentration in tissue by reflectance spectrophotometry,” Photochem. Photobiol. 46, 337–343 (1987). [CrossRef] [PubMed]
  3. M. Cope, D. T. Delpy, “System for long-term measurement of cerebral blood and tissue oxygenation on newborn infants by near infrared transillumination,” Med. Biol. Eng. Comput. 26, 289–294(1988). [CrossRef] [PubMed]
  4. B. C. Wilson, M. S. Patterson, “The physics of photodynamic therapy,” Phys. Med. Biol. 31, 327–360 (1986). [CrossRef] [PubMed]
  5. S. L. Jacques, S. A. Prahl, “Modeling optical and thermal distribution in tissue during laser irradiation,” Laser Surg. Med. 6, 494–503 (1987). [CrossRef]
  6. W. F. Cheong, S. A. Prahl, A. J. Welch, “A review of the optical properties of biological tissues,” IEEE J. Quantum Electron. 26, 2166–2185 (1990). [CrossRef]
  7. F. F. Jobsis, J. H. Keizer, J. C. LaManna, M. Rosenthal, “Reflectance spectrophotometry of cytochrome aa3in vivo,” J. Appl. Physiol. 43, 858–872 (1977). [PubMed]
  8. E. Gratton, “Method for the automatic correction of scattering in absorption spectra by using the integrating sphere,” Biopolymers 10, 2629–2634 (1971). [CrossRef] [PubMed]
  9. R. R. Anderson, J. A. Parrish, “Optical properties of human skin,” in The Science of Photomedicine, J. D. Regan, J. A. Parrish, eds. (Plenum, New York, 1982). [CrossRef]
  10. P. Parsa, S. L. Jacques, N. S. Nishioka, “Optical properties of rat liver between 350 and 2200 nm,” Appl. Opt. 28, 2325–2330 (1989). [CrossRef] [PubMed]
  11. T. J. Farrel, M. S. Patterson, B. Wilson, “A diffusion theory model of spatially resolved, steady-state diffuse reflectance for the noninvasive determination of tissue optical properties in vivo,” Med. Phys. 19, 879–888 (1992). [CrossRef]
  12. K. M. Yoo, Y. Takiguchi, R. R. Alfano, “Dynamic effect of weak localization on the light scattering from random media using ultrafast laser technology,” Appl. Opt. 28, 2343–2349 (1989). [CrossRef] [PubMed]
  13. R. Araki, I. Nashimoto, “Near-infrared imaging in vivo: imaging of Hb oxygenation in living tissues,” in Time-Resolved Spectroscopy and Imaging of Tissues, B. Chance, A. Katzir, eds., Proc. Soc. Photo-Opt. Instrum. Eng.1431, 321–322 (1991).
  14. M. S. Patterson, B. Chance, B. C. Wilson, “Time resolved reflectance and transmittance for the noninvasive measurement of tissue optical properties,” Appl. Opt. 28, 2331–2336 (1989). [CrossRef] [PubMed]
  15. M. S. Patterson, J. D. Moulton, B. C. Wilson, K. W. Berndt, J. R. Lakowicz, “Frequency-domain reflectance for the determination of the scattering and absorption properties of tissue,” Appl. Opt. 30, 4474–4476 (1991). [CrossRef] [PubMed]
  16. J. B. Fishkin, E. Gratton, “Propagation of photon-density waves in strongly scattering media containing an absorbing semi-infinite plane bounded by a straight edge,” J. Opt. Soc. Am. A 10, 127–140 (1993). [CrossRef] [PubMed]
  17. Aldrich Chemical Company Laboratories, Milwaukee, Wisconsin, 1993.
  18. B. A. Feddersen, D. W. Piston, E. Gratton, “Digital parallel acquisition in frequency domain fluorometry,” Rev. Sci. Instrum. 60, 2929–2936 (1989). [CrossRef]
  19. N. E. Dorsey, ed., Properties of Ordinary Water-Substance, (Reinhold, New York, 1940).
  20. H. J. van Staveren, C. J. M. Moes, J. van Marie, S. A. Prahl, M. J. C. van Gemert, “Light scattering in Intralipid-10% in the wavelength range of 400–1100 nm,” Appl. Opt. 30, 4507–4514 (1991). [CrossRef] [PubMed]
  21. J. L. Karagiannes, Z. Zhang, B. Grossweiner, L. I. Grossweiner, “Applications of the 1-D diffusion approximation to the optics of tissues and tissue phantoms,” Appl. Opt. 28, 2311–2317(1989). [CrossRef] [PubMed]
  22. M. S. Patterson, J. D. Moulton, B. C. Wilson, B. Chance, “Applications of time-resolved light scattering measurements to photodynamic therapy dosimetry,” in Photodynamic Therapy: Mechanisms II, T. J. Dougherty, ed., Proc. Soc. Photo-Opt. Instrum. Eng.1203, 62–75 (1990).
  23. L. O. Svaasand, B. J. Tromberg, R. C. Haskell, T.-T. Tsay, M. W. Berns, “Tissue characterization and imaging using photon density waves,” Opt. Eng. 32, 258–266 (1993). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited