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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 33, Iss. 4 — Feb. 1, 1994
  • pp: 702–708

Confocal slit divided-aperture microscope: applications in ear research

Charles J. Koester, Shyam M. Khanna, Heinz D. Rosskothen, Robert B. Tackaberry, and Mats Ulfendahl  »View Author Affiliations


Applied Optics, Vol. 33, Issue 4, pp. 702-708 (1994)
http://dx.doi.org/10.1364/AO.33.000702


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Abstract

A confocal, scanning slit microscope that uses separate portions of the objective aperture for illumination and imaging rays achieves a high degree of optical sectioning. This capability permits visualization of individual cells within the intact inner ear in guinea pigs and cats, and it facilitates directing a laser heterodyne interferometer beam so that vibration of selected cells can be measured. A concentric singlet lens is added to the front of a long-working-distance microscope objective to increase the numerical aperture from 0.4 to 0.53 while retaining a working distance of 6 mm. The measured optical-sectioning capability is compared with the theoretical performance and with the calculated curve for a full-aperture pinhole confocal system.

© 1994 Optical Society of America

History
Original Manuscript: April 6, 1993
Revised Manuscript: September 17, 1993
Published: February 1, 1994

Citation
Charles J. Koester, Shyam M. Khanna, Heinz D. Rosskothen, Robert B. Tackaberry, and Mats Ulfendahl, "Confocal slit divided-aperture microscope: applications in ear research," Appl. Opt. 33, 702-708 (1994)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-33-4-702

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