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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 34, Iss. 12 — Apr. 20, 1995
  • pp: 1989–2003

Fluorescence of coumarins and xanthenes after two-photon absorption with a pulsed titanium–sapphire laser

A. Fischer, C. Cremer, and E. H. K. Stelzer  »View Author Affiliations


Applied Optics, Vol. 34, Issue 12, pp. 1989-2003 (1995)
http://dx.doi.org/10.1364/AO.34.001989


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Abstract

Fluorescence emission after two-photon absorption of coumarins and xanthenes in an alcoholic solution was measured in the tuning range of a femtosecond-pulsed titanium–sapphire laser (750–840 nm). Xanthenes, which have a low one-photon absorption in the near UV, show a higher fluorescence signal after two-photon absorption than the UV-excitable coumarins. When fluxes of 1028 photons/(cm2 s) are used, the two-photon absorption cross sections for xanthenes are 1 order of magnitude higher than the two-photon absorption cross sections of the coumarins. Absolute cross sections have been estimated for three coumarins and three xanthenes. For the xanthenes a significant wavelength-dependent departure from the expected fluorescence intensity square law was observed. The coumarins follow the square-law dependence. The consequences of the findings are discussed for analytic and diagnostic methods. An especially important result is that the resolution in two-photon microscopy of xanthenes is worse than expected.

© 1995 Optical Society of America

History
Original Manuscript: June 3, 1994
Revised Manuscript: September 8, 1994
Published: April 20, 1995

Citation
A. Fischer, C. Cremer, and E. H. K. Stelzer, "Fluorescence of coumarins and xanthenes after two-photon absorption with a pulsed titanium–sapphire laser," Appl. Opt. 34, 1989-2003 (1995)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-34-12-1989


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