An overlap term is used in fluorescence theory to account for the spectrally distributed interaction between laser radiation and molecular transitions. We present a dimensionless overlap fraction formulation. Compared with the more common dimensional overlap term [in units of inverse wave number (1/cm−1)], this form of expression of the interaction between a laser and an absorption transition has a much more practical interpretation and simplifies the equations that describe fluorescence measurements.
© 1995 Optical Society of America
Original Manuscript: September 6, 1994
Revised Manuscript: January 23, 1995
Published: May 20, 1995
William P. Partridge and Normand M. Laurendeau, "Formulation of a dimensionless overlap fraction to account for spectrally distributed interactions in fluorescence studies," Appl. Opt. 34, 2645-2647 (1995)