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Applied Optics

Applied Optics


  • Vol. 34, Iss. 18 — Jun. 20, 1995
  • pp: 3218–3222

Single-molecule detection: applications to ultrasensitive biochemical analysis

Alonso Castro and E. Brooks Shera  »View Author Affiliations

Applied Optics, Vol. 34, Issue 18, pp. 3218-3222 (1995)

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Recent developments in laser-based detection of fluorescent molecules have made possible the implementation of very sensitive techniques for biochemical analysis. We present and discuss our experiments on the applications of our recently developed technique of single-molecule detection to the analysis of molecules of biological interest. These newly developed methods are capable of detecting and identifying biomolecules at the single-molecule level of sensitivity. In one case, identification is based on measuring fluorescence brightness from single molecules. In another, molecules are classified by determining their electrophoretic velocities.

© 1995 Optical Society of America

Original Manuscript: August 19, 1994
Revised Manuscript: November 7, 1994
Published: June 20, 1995

Alonso Castro and E. Brooks Shera, "Single-molecule detection: applications to ultrasensitive biochemical analysis," Appl. Opt. 34, 3218-3222 (1995)

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