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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 34, Iss. 27 — Sep. 20, 1995
  • pp: 6301–6305

Measurement of scattering properties of individual particles with a scanning flow cytometer

A. V. Chernyshev, V. I. Prots, A. A. Doroshkin, and V. P. Maltsev  »View Author Affiliations


Applied Optics, Vol. 34, Issue 27, pp. 6301-6305 (1995)
http://dx.doi.org/10.1364/AO.34.006301


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Abstract

A hydrofocusing head with an optical cuvette has been developed for the flow cytometer to generate complete scatter patterns of single particles at scattering angles ranging from 10° to 120°. The scatter signal has been measured as a function of the angle (a flying indicatrix) by the use of particle motion within a scanning system of the flow cytometer by the use of a single photomultiplier. Scattering data measured with the flow cytometer have been compared with those calculated from Mie theory for latex particles. A calculation algorithm has been used to estimate the size and the refractive index of spherical particles from the scattering data measured.

© 1995 Optical Society of America

History
Original Manuscript: October 28, 1993
Revised Manuscript: December 13, 1994
Published: September 20, 1995

Citation
A. V. Chernyshev, V. I. Prots, A. A. Doroshkin, and V. P. Maltsev, "Measurement of scattering properties of individual particles with a scanning flow cytometer," Appl. Opt. 34, 6301-6305 (1995)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-34-27-6301

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