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Applied Optics

Applied Optics


  • Vol. 34, Iss. 6 — Feb. 20, 1995
  • pp: 977–982

Calibration of light forces in optical tweezers

Harald Felgner, Otto Müller, and Manfred Schliwa  »View Author Affiliations

Applied Optics, Vol. 34, Issue 6, pp. 977-982 (1995)

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Axial and lateral optical-trapping forces on polystyrene and glass microbeads are measured as a function of sphere size and axial trapping position inside a specimen chamber containing water. A strong decrease of the light forces with increasing distance of the trapping position from the coverslip of the chamber is found. It is shown that beyond a certain maximal distance the trapping efficiency decreases substantially but trapping becomes possible in different, axial positions. We consider these effects to be accounted for by spherical aberration of the focused laser beam.

© 1995 Optical Society of America

Original Manuscript: August 1, 1994
Published: February 20, 1995

Harald Felgner, Otto Müller, and Manfred Schliwa, "Calibration of light forces in optical tweezers," Appl. Opt. 34, 977-982 (1995)

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