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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 35, Iss. 10 — Apr. 1, 1996
  • pp: 1793–1795

Measurement method of the refractive index of biotissue by total internal reflection

Hui Li and Shusen Xie  »View Author Affiliations


Applied Optics, Vol. 35, Issue 10, pp. 1793-1795 (1996)
http://dx.doi.org/10.1364/AO.35.001793


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Abstract

A simple method based on total internal reflection is presented for measuring the refractive index of biological tissue. A narrow laser beam and a semicylindrical lens in contact with a tissue specimen are used in the experimental apparatus. The dependence of the internal reflectivity on the angle of incidence is measured to determine the critical angle and therefore the refractive index of tissue. The experimental results for several tissue samples show that the method is reliable and useful for studying tissue optics. The principal advantages of the method are its elimination of scattering effects, suitability to a small in vitro sample, and excellent accuracy.

© 1996 Optical Society of America

History
Original Manuscript: September 27, 1995
Revised Manuscript: November 29, 1995
Published: April 1, 1996

Citation
Hui Li and Shusen Xie, "Measurement method of the refractive index of biotissue by total internal reflection," Appl. Opt. 35, 1793-1795 (1996)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-35-10-1793

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