A laser scanning differential interference contrast microscope using a differential detection method is proposed. This microscope permits observers to control the differential image contrast with a simple operation. Utilizing this function, the observers are capable of obtaining the optimized differential image whose contrast is most favorable for observation. A simple theoretical analysis and experimental considerations are described.
© 1996 Optical Society of America
Original Manuscript: July 27, 1995
Revised Manuscript: December 11, 1995
Published: May 1, 1996
Hiroshi Ooki, Yutaka Iwasaki, and Jun Iwasaki, "Differential interference contrast microscope with differential detection for optimizing image contrast," Appl. Opt. 35, 2230-2234 (1996)