Time-resolved fluorescence-decay measurement and analysis on single cells by flow cytometry
Applied Optics, Vol. 35, Issue 22, pp. 4481-4489 (1996)
http://dx.doi.org/10.1364/AO.35.004481
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Abstract
A novel method is described for the measurement and analysis of fluorescence decays of individual cells and particles in flow. It combines the rapid measurement capabilities of a flow cytometer and the robust measurement and analysis procedures of time-domain fluorescence-lifetime spectroscopy. For excitation we use a cw laser that is pulse modulated by an electro-optic modulator. The characteristics and the repetition rate of the excitation pulses can be easily adjusted to accommodate fluorescence decays with a wide range of lifetimes.
© 1996 Optical Society of America
Citation
Chiranjit Deka and John A. Steinkamp, "Time-resolved fluorescence-decay measurement and analysis on single cells by flow cytometry," Appl. Opt. 35, 4481-4489 (1996)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-35-22-4481
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