OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 35, Iss. 22 — Aug. 1, 1996
  • pp: 4481–4489

Time-resolved fluorescence-decay measurement and analysis on single cells by flow cytometry

Chiranjit Deka and John A. Steinkamp  »View Author Affiliations


Applied Optics, Vol. 35, Issue 22, pp. 4481-4489 (1996)
http://dx.doi.org/10.1364/AO.35.004481


View Full Text Article

Enhanced HTML    Acrobat PDF (404 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

A novel method is described for the measurement and analysis of fluorescence decays of individual cells and particles in flow. It combines the rapid measurement capabilities of a flow cytometer and the robust measurement and analysis procedures of time-domain fluorescence-lifetime spectroscopy. For excitation we use a cw laser that is pulse modulated by an electro-optic modulator. The characteristics and the repetition rate of the excitation pulses can be easily adjusted to accommodate fluorescence decays with a wide range of lifetimes.

© 1996 Optical Society of America

History
Original Manuscript: September 11, 1995
Revised Manuscript: January 2, 1996
Published: August 1, 1996

Citation
Chiranjit Deka and John A. Steinkamp, "Time-resolved fluorescence-decay measurement and analysis on single cells by flow cytometry," Appl. Opt. 35, 4481-4489 (1996)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-35-22-4481

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited