We have designed, fabricated, and tested a compact, transportable, excitation-emission spectrofluorimeter with optical-fiber light delivery and collection for use in rapid analysis of tissues in a clinical setting. This system provides up to eleven different excitation wavelengths, permitting collection of all the corresponding emission spectra in approximately 600 ms. It uses a N2 laser that pumps a sequence of dyes placed in cuvettes on a rotating wheel. A white-light excitation source permits acquisition of the tissue’s diffuse reflectance spectrum on each cycle. Return fluorescence and reflected light are dispersed by a small spectrograph and detected by a photodiode-array detector. The system can collect a single-shot spectrum from biological tissue with a signal-to-noise ratio in excess of 50:1.
© 1996 Optical Society of America
Original Manuscript: November 8, 1995
Revised Manuscript: March 5, 1996
Published: September 1, 1996
Renato Amaro Zângaro, Landulfo Silveira, Ramasamy Manoharan, George Zonios, Irving Itzkan, Ramachandra R. Dasari, Jacques Van Dam, and Michael S. Feld, "Rapid multiexcitation fluorescence spectroscopy system for in vivo tissue diagnosis," Appl. Opt. 35, 5211-5219 (1996)