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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 35, Iss. 25 — Sep. 1, 1996
  • pp: 5220–5226

Imaging acousto-optic tunable filter with 0.35-micrometer spatial resolution

Elliot S. Wachman, Wen-hua Niu, and Daniel L. Farkas  »View Author Affiliations


Applied Optics, Vol. 35, Issue 25, pp. 5220-5226 (1996)
http://dx.doi.org/10.1364/AO.35.005220


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Abstract

Image blur in acousto-optic tunable filters (AOTF’s) has been a persistent problem. Here we describe the connection between transducer structure and image blur and experimentally measure it by using a 5-cm 12°-cut TeO2 crystal of our design. With these quantitative results, we develop an image-processing method that minimizes AOTF-related image degradation. The combination of long crystal design and image processing results in substantially improved image contrast and spatial resolution relative to conventional AOTF imaging devices. We present high-magnification images of fluorescent actin fibers in cells in which we obtain a resolution of approximately 0.35 μm, representing the first successful use of an AOTF for ultra-high-resolution microscopy. Further improvements are also predicted.

© 1996 Optical Society of America

History
Original Manuscript: November 29, 1995
Revised Manuscript: March 4, 1996
Published: September 1, 1996

Citation
Elliot S. Wachman, Wen-hua Niu, and Daniel L. Farkas, "Imaging acousto-optic tunable filter with 0.35-micrometer spatial resolution," Appl. Opt. 35, 5220-5226 (1996)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-35-25-5220

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