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Applied Optics

Applied Optics


  • Vol. 35, Iss. 34 — Dec. 1, 1996
  • pp: 6732–6739

Effect of half-stop lateral misalignment on imaging of dark-field and stereoscopic confocal microscopes

P. Török, Z. Laczik, and C. J. R. Sheppard  »View Author Affiliations

Applied Optics, Vol. 35, Issue 34, pp. 6732-6739 (1996)

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There are several ways to realize dark-field imaging in confocal microscopy. In a recent paper [J. Microsc. 181 260–268 (1996)] we suggested a simple modification of a commercial confocal microscope to incorporate dark-field imaging. This modification involved an aperture stop covering half of the entrance pupil of the objective lens. Now we investigate the lateral misalignment of the aperture stop for dark-field and stereoscopic confocal microscopes. We show the effect of lateral alignment of the half-stop on the point-spread and transfer functions and also examine the detected signal from a sloping plane reflector. Lateral and axial resolution values are given from theoretical data.

© 1996 Optical Society of America

Original Manuscript: January 16, 1996
Revised Manuscript: April 29, 1996
Published: December 1, 1996

P. Török, Z. Laczik, and C. J. R. Sheppard, "Effect of half-stop lateral misalignment on imaging of dark-field and stereoscopic confocal microscopes," Appl. Opt. 35, 6732-6739 (1996)

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  1. P. Török, Z. Laczik, J. N. Skepper, “Simple modification of a commercial scanning laser microscope to incorporate dark-field imaging,” J. Microsc. 181, 260–269 (1996). [CrossRef] [PubMed]
  2. P. Török, Z. Laczik, C. J. R. Sheppard, J. N. Skepper, “Dark-field and differential phase contrast imaging modes in confocal microscopy using a half-aperture stop,” presented at the Conference on 3D Imaging Sciences in Microscopy, Oxford, England, April 1996.
  3. C. J. R. Sheppard, D. K. Hamilton, “High resolution stereoscopic imaging,” Appl. Opt. 22, 866–867 (1983). [CrossRef]
  4. T. Wilson, C. J. R. Sheppard, Theory and Practice of Scanning Optical Microscopy (Academic, London, 1984).
  5. A. R. Carlini, “Imaging modes of scanning confocal microscopy,” D. Phil. dissertation (University of Oxford, Oxford, UK, 1988).

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