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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 36, Iss. 1 — Jan. 1, 1997
  • pp: 125–135

Influence of particle size and concentration on the diffuse backscattering of polarized light from tissue phantoms and biological cell suspensions

Andreas H. Hielscher, Judith R. Mourant, and Irving J. Bigio  »View Author Affiliations


Applied Optics, Vol. 36, Issue 1, pp. 125-135 (1997)
http://dx.doi.org/10.1364/AO.36.000125


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Abstract

We present experimental results that show the spatial variations of the diffuse-backscattered intensity when linearly polarized light is incident upon highly scattering media. Experiments on polystyrene-sphere and Intralipid suspensions demonstrate that the radial and azimuthal variations of the observed pattern depend on the concentration, size, and anisotropy factor g of the particles that constitute the scattering medium. Measurements performed on biological-cell suspensions show the potential of this method for cell characterization.

© 1997 Optical Society of America

History
Original Manuscript: April 17, 1996
Revised Manuscript: August 27, 1996
Published: January 1, 1997

Citation
Andreas H. Hielscher, Judith R. Mourant, and Irving J. Bigio, "Influence of particle size and concentration on the diffuse backscattering of polarized light from tissue phantoms and biological cell suspensions," Appl. Opt. 36, 125-135 (1997)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-36-1-125


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References

  1. W. S. Bickel, J. F. Davidson, D. R. Huffman, R. Kilkson, “Application of polarization effects in light scattering: a new biophysical tool,” Proc. Natl. Acad. Sci. USA 73, 486–490 (1976). [CrossRef] [PubMed]
  2. H. C. van de Hulst, Light Scattering by Small Particles (Wiley, New York, 1957), 40–59.
  3. W. P. van De Merwe, D. R. Huffman, B. V. Bronk, “Reproducibility and sensitivity of polarized-light scattering for identifying bacterial suspensions,” Appl. Opt. 28, 5052–5057 (1989). [CrossRef] [PubMed]
  4. B. V. Bronk, W. P. van de Merwe, M. Stanley, “An in vivo measure of average bacterial cell size from polarized light scattering function,” Cytometry 13, 155–162 (1992). [CrossRef]
  5. B. V. Bronk, S. D. Druger, J. Czege, W. P. van de Merwe, “Measuring diameters of rod-shaped bacteria in vivo with polarized light scattering,” Biophys. J. 69, 1170–1177 (1995). [CrossRef] [PubMed]
  6. W. S. Bickel, M. E. Stafford, “Polarized light scattering from biological systems: a technique for cell differentiation,” J. Biol. Phys. 9, 53–66 (1981). [CrossRef]
  7. B. G. deGrooth, L. W. M. M. Terstappen, G. J. Puppels, J. Greve, “Light-scattering polarization measurements as a new parameter in flow cytometry,” Cytometry 8, 539–544 (1987). [CrossRef]
  8. G. C. Salzman, S. B. Singham, R. G. Johnston, C. F. Bohren, “Light scattering and cytometry,” in Flow Cytometry and Sorting, 2nd ed. (Wiley-Liss, New York, 1990), pp. 81–107.
  9. S. Zeitz, A. Belmont, C. Nicolini, “Differential scattering of circularly polarized light as a unique probe of polynucleosome superstructure,” Cell Biophys. 5, 163–187 (1983). [CrossRef]
  10. I. J. Tinoco, W. Mickols, M. F. Maestre, C. Bustamante, “Absorption, scattering, and imaging of biomolecular structures with polarized light,” Ann. Rev. Biophys. Biochem. 16, 319–349 (1987).
  11. M. I. Mishchenko, J. W. Hovenier, “Depolarization of light backscattered by randomly oriented nonspherical particles,” Opt. Lett. 20, 1356–1358 (1995). [CrossRef] [PubMed]
  12. N. J. McCormick, “Particle-size-distribution retrieval from backscattered polarized radiation measurements: a proposed method,” J. Opt. Soc. Am. A 7, 1811–1816 (1990). [CrossRef]
  13. K. M. Yoo, R. R. Alfano, “Time resolved depolarization of multiple backscattered light from random media,” Phys. Lett. A 142, 531–536 (1989). [CrossRef]
  14. R. R. Anderson, “Polarized-light examination and photography of the skin,” Arch. Dermatol. 127, 1000–1005 (1991). [CrossRef] [PubMed]
  15. L. Wang, D. V. Stephens, S. L. Thomsen, S. L. Jacques, F. K. Tittel, “Polarized diffuse reflectance of laser light on a turbid medium,” presented at the International Conference on Future Directions of Lasers in Surgery and Medicine, Engineering Foundation, Snowbird, Utah, 9–14 July 1995.
  16. S. L. Jacques, L. H. Wang, D. V. Stephens, M. Ostermeyer, “Polarized light transmission through skin using video reflectometry: toward optical tomography of superficial tissue layers,” in Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems VI, R. R. Anderson, ed., Proc. SPIE2671, 199–220 (1996).
  17. T. J. Farrell, M. S. Patterson, B. Wilson, “A diffusion theory model of spatially resolved, steady-state diffuse reflectance for the noninvasive determination of tissue optical properties in vivo,” Med. Phys. 19, 879–888 (1992). [CrossRef] [PubMed]
  18. M. Dogariu, T. Asakura, “Photon pathlength distribution from polarized backscattering in random media,” Opt. Eng. 35, 2234–2239 (1996). [CrossRef]
  19. W. M. Star, J. P. A. Marijnissen, H. Jansen, M. Keijzer, M. J. C. van Gemert, “Light dosimetry for photodynamic therapy by whole bladder wall irradiation,” Photchem. Photobiol. 46, 619–624 (1987). [CrossRef]
  20. C. J. M. Moes, M. J. C. van Gemert, W. M. Star, J. P. A. Marijnissen, S. A. Prahl, “Measurements and calculations of the energy fluence rate in a scattering and absorbing phantom at 633 nm,” Appl. Opt. 28, 2292–2296 (1989). [CrossRef] [PubMed]
  21. H. J. van Staveren, C. J. M. Moes, J. van Marle, S. A. Prahl, M. J. C. van Gemert, “Light scattering in Intralipid-10% in the wavelength range of 400–1100 nm,” Appl. Opt. 30, 4507–4514 (1991). [CrossRef] [PubMed]
  22. S. T. Flock, S. L. Jacques, B. C. Wilson, W. M. Star, M. J. C. van Gemert, “Optical properties of Intralipid: a phantom medium for light propagation studies,” Lasers Surg. Med. 12, 510–519 (1992). [CrossRef] [PubMed]
  23. W. H. Press, B. P. Flannery, S. A. Teukolsky, W. T. Vetterling, Numerical Recipes in C (Cambridge U. Press, New York, 1995), p. 611.
  24. B. Beauvoit, H. Liu, K. Kang, P. D. Kaplan, M. Miwa, B. Chance, “Characterization of absorption and scattering properties for various yeast strains by time-resolved spectroscopy,” Cell Biophys. 23, 91–109 (1994). [CrossRef]
  25. L. C. Junqueira, J. Carneiro, R. O. Kelley, Basic HistologyAppleton & Lange, Norwalk, Conn., 1992), pp. 108–110.
  26. J. P. Freyer, “Rates of oxygen consumption for proliferating and quiescent cells isolated from multicellular tumor spheroids,” Adv. Exp. Med. Biol. 345, 335–342 (1994). [CrossRef] [PubMed]
  27. W. K. Pratt, Digital Image Processing (Wiley, New York, 1991), pp. 285–302.
  28. A. K. Jain, Fundamentals of Digital Image Processing (Prentice-Hall, Englewood Cliffs, N.J., 1989), pp. 241–242.
  29. C. F. Bohren, D. R. Huffman, Absorption and Scattering of Light by Small Particles (Wiley, New York, 1983), pp. 82–129.
  30. A. H. Cook, The Chemistry and Biology of Yeast (Academic, New York, 1958), p. 235.
  31. E. A. Munn, The Structure of Mitochondria (Academic, New York, 1974).
  32. B. Beauvoit, T. Kitai, B. Chance, “Contribution of the mitochondrial compartment to the optical properties of the rat liver: a theoretical and practical approach,” Biophys. J. 67, 2501–2510 (1994). [CrossRef] [PubMed]
  33. F. C. MacKintosh, J. X. Zhu, D. J. Pine, D. A. Weitz, “Polarization memory of multiply scattered light,” Phys. Rev. B 40, 9342–9345 (1989). [CrossRef]
  34. A. Brunsting, P. F. Mullaney, “Differential light scattering from spherical mammalian cells,” Biophys. J. 14, 439–453 (1974). [CrossRef] [PubMed]
  35. S. Fujime, M. Takasaki-Oshito, S. Miyamoto, “Dynamic light scattering from polydisperse suspensions of large spheres,” Biophys. J. 54, 1179–1184 (1988). [CrossRef] [PubMed]
  36. R. C. Weast, ed., CRC Handbook of Chemistry and Physics (CRC Press, Boca Raton, Fla., 1989).
  37. F. A. Duck, Physical Properties of Tissue (Academic, London, 1990), p. 63.
  38. H. Li, S. Xie, “Measurement method of the refractive index of biotissue by total internal reflection,” Appl. Opt. 35, 1793–1795 (1996). [CrossRef] [PubMed]

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