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Applied Optics

Applied Optics


  • Vol. 36, Iss. 1 — Jan. 1, 1997
  • pp: 397–401

Measurement of group delay dispersion of high numerical aperture objective lenses using two-photon excited fluorescence

Jeffrey B. Guild, Chris Xu, and Watt W. Webb  »View Author Affiliations

Applied Optics, Vol. 36, Issue 1, pp. 397-401 (1997)

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We determined the group-delay dispersion (GDD) of five microscope objectives by measuring the second-order autocorrelation at the focal points of the objectives with two-photon excited fluorescence as the power square sensor. We found that typical microscope lens systems introduce significant GDD (2000–6500 fs2). The third-order dispersion determined for these objectives limits the minimum obtainable pulse width at the focal point of an objective to 20–30 fs if not compensated. No significant chromatic aberration or higher-order dispersion effects were found for any of the optical components measured within the wavelength range of 700–780 nm and for pulse widths greater than 50–60 fs.

© 1997 Optical Society of America

Original Manuscript: November 27, 1995
Revised Manuscript: July 9, 1996
Published: January 1, 1997

Jeffrey B. Guild, Chris Xu, and Watt W. Webb, "Measurement of group delay dispersion of high numerical aperture objective lenses using two-photon excited fluorescence," Appl. Opt. 36, 397-401 (1997)

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