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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 36, Iss. 12 — Apr. 20, 1997
  • pp: 2481–2483

Two-photon fluorescence microscopy with a diode-pumped Cr:LiSAF laser

G. Robertson, D. Armstrong, M. J. P. Dymott, A. I. Ferguson, and G. L. Hogg  »View Author Affiliations


Applied Optics, Vol. 36, Issue 12, pp. 2481-2483 (1997)
http://dx.doi.org/10.1364/AO.36.002481


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Abstract

We demonstrate the acquisition of high-quality two-photon fluorescence microscopy images using an all-solid-state self-mode-locked Cr:LiSAF laser. We contrast the performance of the two-photon technique with single-photon confocal fluorescence microscopy images taken with an argon-ion laser. Examples of improved depth penetration and reduced dye bleaching are presented.

© 1997 Optical Society of America

History
Original Manuscript: February 1, 1996
Revised Manuscript: September 9, 1996
Published: April 20, 1997

Citation
G. Robertson, D. Armstrong, M. J. P. Dymott, A. I. Ferguson, and G. L. Hogg, "Two-photon fluorescence microscopy with a diode-pumped Cr:LiSAF laser," Appl. Opt. 36, 2481-2483 (1997)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-36-12-2481

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