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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 36, Iss. 22 — Aug. 1, 1997
  • pp: 5608–5620

Clinical imaging fluorescence apparatus for the endoscopic photodetection of early cancers by use of Photofrin II

Georges A. Wagnières, André P. Studzinski, Daniel R. Braichotte, Philippe Monnier, Christian Depeursinge, André Châtelain, and Hubert E. van den Bergh  »View Author Affiliations


Applied Optics, Vol. 36, Issue 22, pp. 5608-5620 (1997)
http://dx.doi.org/10.1364/AO.36.005608


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Abstract

A fluorescence imaging device applied to the detection of early cancer is described. The apparatus is based on the imaging of laser-induced fluorescence of a dye that localizes in a tumor with a higher concentration than in the surrounding normal tissue after iv injection. Tests carried out in the upper aerodigestive tract, the tracheobronchial tree, and the esophagus with Photofrin II (1 mg/kg of body weight) as the fluorescent agent are reported as examples. The fluorescence is induced by violet (410-nm) light from a continuous-wave (cw) krypton-ion laser. The fluorescence contrast between tumor and surrounding tissue is enhanced by real-time image processing. This is done by the simultaneous recording of the fluorescence image in two spectral domains (470–600 and 600–720 nm), after which these two images are digitized and manipulated with a mathematical operator (look-up table) at video frequency. Among the 7 photodetections performed in the tracheobronchial tree, 6 were successful, whereas it was the case for only 5 of the 15 lesions investigated in squamous mucosa (upper aerodigestive tract and esophagus). The sources of false positives and false negatives are evaluated in terms of the fluorescent dye, tissue optical properties, and illumination optics.

© 1997 Optical Society of America

History
Original Manuscript: May 14, 1996
Revised Manuscript: January 9, 1997
Published: August 1, 1997

Citation
Georges A. Wagnières, André P. Studzinski, Daniel R. Braichotte, Philippe Monnier, Christian Depeursinge, André Châtelain, and Hubert E. van den Bergh, "Clinical imaging fluorescence apparatus for the endoscopic photodetection of early cancers by use of Photofrin II," Appl. Opt. 36, 5608-5620 (1997)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-36-22-5608


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References

  1. R. T. Osteen, Cancer Manual, 8th ed. [American Cancer Society (Massachussetts Division), Boston, 1990].
  2. P. C. Norwell, “Mechanisms of tumor progression,” Cancer Res. 46, 2203–2207 (1986).
  3. R. Pasche, M. Savary, Ph. Monnier, “Multiple squamous cell carcinoma of the upper digestive and lower respiratory tract: methodology of endoscopic screening,” Acta Endosc. 11, 277–291 (1981). [CrossRef]
  4. T. Muto, J. R. Buessey, B. C. Morson, “The evaluation of cancer of the colon and rectum,” Cancer 36, 2251–2270 (1975). [CrossRef] [PubMed]
  5. S. Undenfriend, Fluorescence Assay in Biology and Medicine (Academic, New York, 1962), Vol. I.
  6. R. Richards-Kortum, E. Sevick-Muraca, “Quantitative Optical Spectroscopy for Tissue Diagnosis,” Ann. Rev. Phys. Chem. 47, 555–606 (1996). [CrossRef]
  7. A. E. Profio, D. R. Doiron, “Laser fluorescence bronchoscopy for localization of small lung tumors,” Phys. Med. Biol. 22, 949–957 (1977). [CrossRef] [PubMed]
  8. R. R. Alfano, D. B. Tata, J. J. Cordero, P. Tomashefsky, F. W. Longo, M. A. Alfano, “Laser induced fluorescence spectroscopy from native cancerous and normal tissue,” IEEE J. Quantum Electron. QE-20, 1507–1511 (1984). [CrossRef]
  9. W. Lohmann, “In situ detection of melanomas by fluorescence measurements,” Naturwissenschaften 75, 201–202 (1988). [CrossRef] [PubMed]
  10. Y. Yang, Y. Ye, F. Li, Y. Li, P. Ma, “Characteristic autofluorescence for cancer diagnosis and its origin,” Lasers Surg. Med. 7, 528–532 (1987). [CrossRef] [PubMed]
  11. D. Braichotte, G. Wagnières, Ph. Monnier, M. Savary, R. Bays, H. van den Bergh, A. Châtelain, “Endoscopic tissue autofluorescence measurements in the upper aerodigestive tract and the bronchi,” in Future Trends in Biomedical Applications of Lasers, L. O. Svaasand, ed., Proc. SPIE1525, 211–218 (1991).
  12. R. R. Alfano, A. Pradhan, G. C. Tang, “Optical spectroscopic diagnosis of cancer and normal breast tissues,” J. Opt. Soc. Am. B 6, 1015–1023 (1989). [CrossRef]
  13. P. N. Yashke, R. F. Bonner, P. Cohen, M. B. Leon, D. E. Fleischer, “Laser induced fluorescence spectroscopy may distinguish colon cancer from normal human colon,” Gastrointest. Endosc. 35, 184 (1989).
  14. C. R. Kapadia, F. W. Cutruzolla, K. M. O’Brian, M. L. Stetz, R. Enriquez, L. I. Deckelbaum, “LIF spectroscopy of human colonic mucosa,” Gastroenterology 99, 150–157 (1990). [PubMed]
  15. R. Marchesini, M. Brambilla, E. Pigoli, G. Bottiroli, A. C. Croce, M. Del Fante, P. Spinelli, S. Di Palma, “Light-induced fluorescence spectroscopy of adenoma, adenocarcinomas and non-neoplastic mucosa in human colon. I: in vitro measurements,” J. Photochem. Photobiol. B 14, 219–230 (1992). [CrossRef] [PubMed]
  16. K. T. Schomacker, J. K. Frisoli, C. C. Compton, T. J. Flotte, J. M. Richter, N. S. Nishioka, T. F. Deutsch, “Ultraviolet laser-induced fluorescence of colonic tissue: basic biology and diagnostic potential,” Lasers Surg. Med. 12, 63–78 (1992). [CrossRef] [PubMed]
  17. B. Palcic, S. Lam, J. Hung, C. MacAulay, “Detection and localization of early lung cancer by imaging techniques,” Chest 99, 742–743 (1991). [CrossRef] [PubMed]
  18. W. S. Poon, K. T. Schomaker, T. F. Deutsch, R. L. Marturza, “Laser-induced fluorescence (LIF): for intraoperative delineation of tumor resection margins,” J. Neurosurg. 76, 679–686 (1992). [CrossRef] [PubMed]
  19. R. Richards-Kortum, R. P. Rava, R. E. Petras, M. Fitzmauric, M. Sivak, M. Feld, “Spectroscopic diagnosis of colonic dysplasia,” Photochem. Photobiol. 53, 777–786 (1991). [PubMed]
  20. M. Panjehpour, B. F. Overholt, J. L. Schmidhammer, C. Farris, P. F. Buckley, T. Vodinh, “Spectroscopic diagnosis of esophageal cancer—new classification model, improved measurement system,” Gastrointest. Endosc. 41, 577–581 (1995). [CrossRef] [PubMed]
  21. T. Vodinh, M. Panjehpour, B. F. Overholt, C. Farris, F. P. Buckley, R. Sneed, “In Vivo cancer-diagnosis of the esophagus using differential normalized fluorescence (Dnf) indexes,” Lasers Surg. Med. 16, 41–47 (1995). [CrossRef]
  22. M. Panjehpour, B. F. Overholt, T. Vodinh, R. C. Haggitt, D. H. Edwards, F. P. Buckley, “Endoscopic fluorescence detection of high-grade dysplasia in Barretts-esophagus,” Gastroenterology 111, 93–101 (1996). [CrossRef] [PubMed]
  23. J. N. Qu, C. MacAulay, S. Lam, B. Palcic, “Laser-induced fluorescence spectroscopy at endoscopy—tissue optics, Monte-Carlo modeling, and in-vivo measurements,” Opt. Eng. 34, 3334–3343 (1995). [CrossRef]
  24. M. L. Harries, S. Lam, C. MacAulay, J. A. Qu, B. Palcic, “Diagnostic-imaging of the larynx—autofluorescence of laryngeal tumors using the helium cadmium laser,” J. Laryngol. Otol. 109, 108–110 (1995). [CrossRef] [PubMed]
  25. H. J. C. M. Sterenborg, M. Motamedi, R. F. Wagner, M. Duvic, S. Thomsen, S. L. Jacques, “In-vivo fluorescence spectroscopy and imaging of human skin tumors,” Lasers Med. Sci. 9, 191–201 (1994). [CrossRef]
  26. G. Bottiroli, A. C. Croce, D. Locatelli, R. Marchesini, E. Pignoli, S. Tomatis, C. Cuzzoni, S. Di Palma, M. Dalfante, P. Spinelli, “Natural fluorescence of normal and neoplastic human colon—a comprehensive ex-vivo study,” Lasers Surg. Med. 16, 48–60 (1995). [CrossRef]
  27. R. A. Zangaro, L. Silveira, R. Manoharan, G. Zonios, I. Itzkan, R. R. Dasari, J. Van Dam, M. S. Feld, “Rapid multiexcitation fluorescence spectroscopy system for in vivo tissue diagnosis,” Appl. Opt. 35, 5211–5219 (1996). [CrossRef] [PubMed]
  28. A. G. Bohorfoush, “Tissue spectroscopy for gastrointestinal diseases,” Endoscopy 28, 372–380 (1996). [CrossRef] [PubMed]
  29. R. L. Lipson, “The photodynamic and fluorescent properties of a particular hematoporphyrin derivative and its use in tumor detection,” Master’s thesis (University of Minnesota, Minneapolis, Minn., 1960).
  30. D. R. Doiron, A. E. Profio, “Laser fluorescence bronchoscopy for early lung cancer localization,” in Lasers in Photo-medicine and Photobiology, E. Pratesi, C. A. Sacchi, eds. (Springer-Verlag, New York, 1978), pp. 92–95.
  31. D. R. Doiron, A. E. Profio, R. G. Vincent, T. J. Dougherty, “Fluorescence bronchoscopy for localization of occult lung tumors,” Chest 76, 27–32 (1979). [CrossRef] [PubMed]
  32. O. J. Balchum, D. R. Doiron, A. E. Profio, G. C. Huth, “Fluorescence bronchoscopy for localizing early bronchial cancer and carcinoma in situ,” Recent Results Cancer Res. 82, 97–102 (1982). [CrossRef] [PubMed]
  33. A. E. Profio, “Laser excited fluorescence of hematoporphyrin derivative for diagnosis of cancer,” IEEE J. Quantum Electron. QE-20, 1502–1507 (1984). [CrossRef]
  34. R. C. Benson, G. M. Farrow, J. H. Kinsey, D. A. Cortese, H. Zincke, D. C. Utz, “Detection and localization of in situ carcinoma of the bladder with hematoporphyrin derivative,” Mayo Clin. Proc. 57, 548–555 (1982). [PubMed]
  35. D. R. Kinsey, D. R. Cortese, D. R. Sanderson, “Detection of hematoporphyrin fluorescence during fiberoptic bronchoscopy to localize early bronchogenic carcinoma,” Mayo Clin. Proc. 53, 594–600 (1978). [PubMed]
  36. G. Wagnières, Ch. Depeursinge, Ph. Monnier, M. Savary, P. Cornaz, A. Châtelain, H. van den Bergh, “Photodetection of early cancer by laser induced fluorescence of a tumor-selective dye: apparatus design and realization,” in Photodynamic Therapy: Mechanisms II, T. J. Dougherty, ed., Proc. SPIE1203, 43–52 (1990).
  37. A. E. Profio, O. J. Balchum, F. Cartens, “Digital background subtraction for fluorescence imaging,” Med. Phys. 13, 717–721 (1986). [CrossRef] [PubMed]
  38. T. Hirano, M. Ishizuka, K. Suzuki, K. Ishida, S. Suzuki, S. Miyaki, A. Honma, M. Suzuki, K. Aizawa, H. Kato, Y. Hayata, “Photodynamic cancer diagnosis and treatment system consisting of pulsed lasers and endoscopic spectro-image analyzer,” Lasers Life Sci. 3, 99–116 (1989).
  39. R. Baumgartner, H. Fisslinger, D. Jocham, H. Lenz, L. Ruprecht, H. Stepp, E. Unsöld, “A fluorescence imaging device for endoscopic detection of early stage cancer. Instrumental and experimental studies,” Photochem. Photobiol. 46, 759–763 (1987). [CrossRef] [PubMed]
  40. S. Montan, K. Svanberg, S. Svanberg, “Multicolor imaging and contrast enhancement in cancer-tumor localization using laser-induced fluorescence in hematoporphyrin-derivative-bearing tissue,” Opt. Lett. 10, 56–58 (1985). [CrossRef] [PubMed]
  41. G. Wagnières, D. Braichotte, A. Chatelain, Ch. Depeursinge, Ph. Monnier, M. Savary, Ch. Fontolliet, J.-M. Calmes, J.-C. Givel, G. Chapuis, S. Folli, A. Pèlegrin, F. Buchegger, J.-P. Mach, H. van den Bergh, “Photodetection of early cancer in the upper aerodigestive tract and the bronchi using photofrin II and colorectal adenocarcinoma with fluoresceinated monoclonal antibodies,” in Future Trends in Biomedical Applications of Lasers, L. O. Svaasand, ed., Proc. SPIE1525, 219–236 (1991). [CrossRef]
  42. G. H. M. Gijsbers, D. Breederveld, M. C. J. van Gemert, T. A. Boon, J. Langelaar, R. P. H. Rettschnick, “In vivo fluorescence excitation and emission spectra of hematoporphyrin-derivative,” Lasers Life Sci. 1, 29–48 (1986).
  43. Ph. Monnier, M. Savary, Ch. Fontolliet, G. Wagnières, A. Châtelain, P. Cornaz, Ch. Depeursinge, H. van den Bergh, “Photodetection and photodynamic therapy of “early” squamous cell carcinomas of the pharynx, oesophagus and tracheobronchial tree,” Lasers Med. Sci. 5, 149–169 (1990). [CrossRef]
  44. J. C. Kennedy, R. H. Pottier, “Endogenous protoporphyrin IX, a clinically useful photosensitizer for photodynamic therapy,” J. Photochem. Photobiol. 14, 275–292 (1992). [CrossRef]
  45. M. Forrer, Th. Glanzmann, J. Mizeret, D. Braichotte, G. Wagnières, H. van den Bergh, P. Jichlinski, H.-J. Leisinger, “Fluorescence excitation and emission spectra of ALA induced protoporphyrin IX in normal and tumoral tissue of the human bladder,” in Optical Biopsy and Fluorescence Spectroscopy and Imaging, R. Cubeddu, R. Marchesini, S. R. Mordon, K. Svanberg, H. H. Rinneberg, G. Wagnières, eds., Proc. SPIE2324, 84–88 (1995). [CrossRef]
  46. P. Jichlinski, H.-J. Leisinger, M. Forrer, J. Mizeret, Th. Glanzmann, G. Wagnières, H. van den Bergh, L. Guilloud, K. Weber-Chapuis, C. Schindler, A. Pannatier, F. Schmidlin, J. F. Bolle, P. Graber, “Clinical evaluation of a screening method of bladder transitional cell carcinoma by induced fluorescence of Protoporphyrin IX with topical application of delta-aminolevulinic acid. Preliminary results,” Lasers Surg. Med. 20, 402–408 (1997). [CrossRef]
  47. M. Kriegmair, R. Baumgartner, R. Knuechel, P. Steinbach, A. Ehsan, W. Lumper, F. Hofstädter, A. Hofstetter, “Fluorescence photodetection of neoplastic urothelial lesions following instillation of 5-aminolevulinic acid,” Urology 44, 836–841 (1994). [CrossRef] [PubMed]
  48. M. Kriegmair, R. Baumgartner, R. Knuchel, H. Stepp, F. Hofstädter, A. Hofstetter, “Detection of early bladder cancer by 5-aminolevulinic acid-induced porphyrin fluorescence,” J. Urol. 155, 105–109 (1996). [CrossRef]
  49. D. Braichotte, J.-F. Savary, G. Wagnières, A. Mudry, Ch. Fontolliet, Ph. Monnier, H. van den Bergh, “A clinical comparison of the pharmacokinetics of m-THPC and Photofrin II as observed by light induced fluorescence,” in Optical Biopsy, R. Cubbedu, S. Svanberg, H. van den Bergh, eds., Proc. SPIE2081, 62–73 (1994). [CrossRef]
  50. D. Braichotte, J.-F. Savary, P. Westermann, T. Glanzmann, S. Folli, G. Wagnières, Ph. Monnier, H. van den Bergh, “Clinical pharmacokineticstudiesoftetra(metahydroxyphenyl)-chlorin in squamous cell carcinoma by fluorescence spectroscopy at two wavelengths,” Int. J. Cancer 63, 198–204 (1995). [CrossRef] [PubMed]
  51. S. Folli, G. Wagnières, A. Pèlegrin, J.-M. Calmes, D. Braichotte, F. Buchegger, Y. Chalandon, J.-C. Givel, N. Hardman, Ch. Heusser, G. Chapuis, A. Châtelain, H. van den Bergh, J.-P. Mach, “Immunophotodiagnosis of colon carcinomas in patients injected with fluoresceinated chimeric antibodies against carcinoembryonic antigen,” Proc. Natl. Acad. Sci. 89, 7973–7977 (1992).
  52. D. E. Pearson, Transmission and Display of Pictorial Information (Pentech, London, 1975).
  53. G. Wagnières, Ph. Monnier, M. Savary, P. Cornaz, A. Châtelain, H. van den Bergh, “Photodynamic therapy of early cancer in the upper aerodigestive tract and bronchi. Instrumentation and clinical results,” in Future Directions and Applications in Photodynamic Therapy, C. J. Gomer, ed., Vol. IS06 of SPIE Institutes Series (Society of Photo-Optical Instrumentation Engineers, Bellingham, Wash., 1990), pp. 249–271.
  54. D. F. Barbe, S. B. Campana, “Imaging arrays using the charge-coupled concept,” in Advances in Image Pickup and Display, B. Kazan, ed. (Academic, New York, 1977), Vol. 3, p. 171.
  55. D. Braichotte, G. Wagnières, R. Bays, Ph. Monnier, H. van den Bergh, “Clinical pharmacokinetic studies of Photofrin by fluorescence spectroscopy in the oral cavity, the esophagus and the bronchi,” Cancer 75, 2768–2778 (1995). [CrossRef] [PubMed]
  56. M. Kohl, U. Sukowski, B. Ebert, J. Neukammer, H. Rinneberg, Ch. Nowak, “Imaging of superficial growing tumors by delayed observation of laser-induced fluorescence,” in Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy II, T. J. Dougherty, ed., Proc. SPIE1881, 206–221 (1993).
  57. S. Andersson-Engels, J. Johansson, S. Svanberg, “Medical diagnostic system based on simultaneous multispectra fluorescence imaging,” Appl. Opt. 33, 8022–8029 (1994). [CrossRef] [PubMed]
  58. A. M. Mandard, J. Tourneux, M. Gignouxet, L. Blanc, P. Segol, J.-C. Mandard, “In situ carcinoma of the oesophagus: macroscopic study with particular reference to the Lugol test,” Endoscopy 12, 51–57 (1980). [CrossRef] [PubMed]
  59. P. Herlin, J. Marnay, J. H. Jacob, J. M. Ollivier, A. M. Mandard, “A study of the mechanism of the Toluidine Blue dye test,” Endoscopy 15, 4–7 (1983). [CrossRef] [PubMed]
  60. R. Bays, G. Wagnières, D. Robert, D. Braichotte, J.-F. Savary, Ph. Monnier, H. van den Bergh, “Clinical determination of tissue optical properties by endoscopic spatially resolved reflectometry,” Appl. Opt. 35, 1756–1766 (1996). [CrossRef] [PubMed]
  61. H. H. Hopkins, “Optical principles of the endoscope,” in Endoscopy, G. Berci, ed. (Appleton-Century-Crofts, New York, 1976), pp. 3–63.
  62. G. Wagnières, S. Folli, D. Braichotte, H. van den Bergh, “Measurement of the Photofrin II absolute fluorescence quantum yield in tissue,” Photochem. Photobiol. 59, 81S (1994).
  63. S. Lam, C. MacAulay, J. C. LeRiche, J. Qu, G. Krosl, H. Zeng, M. Korbelik, B. Palcic, “Fluorescence imaging of pre-malignant and malignant tissues with and without photosensitizers,” in Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy II, T. J. Dougherty, ed., Proc. SPIE1881, 160–167 (1993).

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