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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 36, Iss. 25 — Sep. 1, 1997
  • pp: 6539–6547

Sensitivity and detection limit of concentration and adsorption measurements by laser-induced surface-plasmon resonance

A. A. Kolomenskii, P. D. Gershon, and H. A. Schuessler  »View Author Affiliations


Applied Optics, Vol. 36, Issue 25, pp. 6539-6547 (1997)
http://dx.doi.org/10.1364/AO.36.006539


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Abstract

The shot noise limitation as well as other factors that influence the sensitivity of measurements with a surface plasmon resonance (SPR) sensor are considered. It is demonstrated that minute changes in the refractive index of a medium close to the surface of a metal film can be detected owing to a shift in the resonance angle. In particular, changes in the adsorption layer of only a fraction of a biomolecular monolayer could be measured. Data for SPRare presented with adjacent media of air, water, as well as aqueous solutions of ethanol and sodium chloride at different concentrations. The immobilization of the protein bovine serum albumin to a specially prepared surface was monitored with the SPR technique. Specific responses to changes in the concentration and thickness of the adsorption layer were determined. The angular resolution of the present apparatus is approximately 1 millidegree, corresponding to a detection limit for an adsorbed protein layer of 15pg/mm2, which is still 2 to 3 orders of magnitude larger than the shot-noise limit, and therefore a further improvement in sensitivity is possible.

© 1997 Optical Society of America

History
Original Manuscript: November 25, 1996
Revised Manuscript: February 10, 1997
Published: September 1, 1997

Citation
A. A. Kolomenskii, P. D. Gershon, and H. A. Schuessler, "Sensitivity and detection limit of concentration and adsorption measurements by laser-induced surface-plasmon resonance," Appl. Opt. 36, 6539-6547 (1997)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-36-25-6539

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