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Applied Optics

Applied Optics


  • Vol. 36, Iss. 7 — Mar. 1, 1997
  • pp: 1681–1683

Near-field optical imaging of flagellar filaments of salmonella in water with optical feedback control

M. Naya, R. Micheletto, S. Mononobe, R. Uma Maheswari, and M. Ohtsu  »View Author Affiliations

Applied Optics, Vol. 36, Issue 7, pp. 1681-1683 (1997)

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We report on the high-resolution observation of biological samples in water with a collection-mode near-field optical microscope (c-mode NOM) operating under optical feedback control. With rapidly decreasing evanescent field power used as the feedback signal, for the first time to our knowledge, an image of straight-type flagellar filaments of salmonella in water has been obtained. The estimated diameter of a single filament is around 55 nm with a pixel size of 10 nm. A comparison with its nominal value of 25 nm obtained from electron microscope observations under high vacuum confirms that our c-mode NOM performs high-resolution imaging in water.

© 1997 Optical Society of America

Original Manuscript: March 26, 1996
Revised Manuscript: November 12, 1996
Published: March 1, 1997

M. Naya, R. Micheletto, S. Mononobe, R. Uma Maheswari, and M. Ohtsu, "Near-field optical imaging of flagellar filaments of salmonella in water with optical feedback control," Appl. Opt. 36, 1681-1683 (1997)

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