Two-Dimensional Visualization of Fluorescence Lifetimes by use of a Picosecond Laser and a Streak Camera
Applied Optics, Vol. 37, Issue 12, pp. 2303-2314 (1998)
http://dx.doi.org/10.1364/AO.37.002303
Acrobat PDF (2339 KB)
Abstract
Two-dimensional distributions of the effective lifetime of the fluorescence emission induced by short-pulsed laser radiation are obtained from two-dimensional images recorded with a streak camera and a charge-coupled device by means of a separation algorithm method (SAM). In theory, the best response with respect to noise is obtained for lifetimes corresponding to a range of pixels of 5–50 in the CCD, that is, 5–50 ps at the fastest streak speed. In experiments the SAM is compared with pure time-resolved measurements, and it is used for two-dimensional lifetime evaluation. The laser-pulse duration is 25 ps, and the lower limit of the lifetime resolution as used in the experiments is estimated to be 200–250 ps. The results demonstrate the possibility of performing pattern recognition independently of the relative distribution of emission intensity between regions of different fluorescence lifetimes. The technique is demonstrated for static objects but can in principle be extended to nonstationary objects if two detectors are used.
© 1998 Optical Society of America
OCIS Codes
(110.0110) Imaging systems : Imaging systems
(300.2530) Spectroscopy : Fluorescence, laser-induced
(320.7150) Ultrafast optics : Ultrafast spectroscopy
Citation
Frederik Ossler, Thomas Metz, Lars Martinsson, and Marcus Aldén, "Two-Dimensional Visualization of Fluorescence Lifetimes by use of a Picosecond Laser and a Streak Camera," Appl. Opt. 37, 2303-2314 (1998)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-37-12-2303
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
Related Journal Articles 
- Narrow-band resonance-ionization and fluorescence imaging in a mercury-vapor cell (OL)
- Picosecond Investigation of the Collisional Deactivation of OH A 2Σ+(v′ = 1, N′ = 4, 12) in an Atmospheric-Pressure Flame (AO)
- Localization of Luminescent Inhomogeneities in Turbid Media with Spatially Resolved Measurements of cw Diffuse Luminescence Emittance (AO)
- Comparison of Nanosecond and Picosecond Excitation for Two-Photon Laser-Induced Fluorescence Imaging of Atomic Oxygen in Flames (AO)
- Comparison of Nanosecond and Picosecond Excitation for Two-Photon Laser-Induced Fluorescence Imaging of Atomic Oxygen in Flames: Erratum (AO)
Related Conference Papers
- Nanoscale Imaging with Coherent Acoustic Phonons Pulses
- Nanostructure imaging with sub-THz coherent acoustic phonon pulses
- Imaging nanostructures with picosecond ultrasonic pulses
- Surface Plasmon Polariton-Based Coaxial Probe for Terahertz Near-Field Microscopy
- Imaging Sensors that asks 20 questions: Fulfilling Dr. Healy’s Vision
- Imaging Sensors that asks 20 questions: Fulfilling Dr. Healy’s Vision
« Previous Article | Next Article »
OSA is a member of 