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Applied Optics

Applied Optics


  • Vol. 37, Iss. 12 — Apr. 20, 1998
  • pp: 2315–2326

Monte Carlo simulation of a single-molecule detection experiment

Dennis H. Bunfield and Lloyd M. Davis  »View Author Affiliations

Applied Optics, Vol. 37, Issue 12, pp. 2315-2326 (1998)

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The physical and instrumental processes that occur in experiments for the detection of individual fluorescent molecules in solution are described, with emphasis on their incorporation into a quantitative Monte Carlo simulation. The simulation is applied to the conditions of a past experiment [Appl. Opt. 34, 3208 (1995)], which utilizes a sheath flow system for high detection efficiency, and it generates comparable results, while helping to identify experimental limitations. The simulation indicates that the use of low dead-time electronics and appropriate selection of experimental parameters should enable detection at more rapid rates for applications in which large numbers of molecules are to be efficiently counted.

© 1998 Optical Society of America

OCIS Codes
(040.1880) Detectors : Detection
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(300.2530) Spectroscopy : Fluorescence, laser-induced

Original Manuscript: July 17, 1997
Revised Manuscript: December 18, 1997
Published: April 20, 1998

Dennis H. Bunfield and Lloyd M. Davis, "Monte Carlo simulation of a single-molecule detection experiment," Appl. Opt. 37, 2315-2326 (1998)

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  23. One significant application currently under development that demands efficient and unambiguous SMD is rapid DNA sequencing. Also, efficient processing of the sample is desirable for assay of miniscule sample quantities, such as the components of a single cell and genetic screening without DNA amplification by polymerase chain reaction. See references of Ref. 1.
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