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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 37, Iss. 12 — Apr. 20, 1998
  • pp: 2315–2326

Monte Carlo simulation of a single-molecule detection experiment

Dennis H. Bunfield and Lloyd M. Davis  »View Author Affiliations


Applied Optics, Vol. 37, Issue 12, pp. 2315-2326 (1998)
http://dx.doi.org/10.1364/AO.37.002315


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Abstract

The physical and instrumental processes that occur in experiments for the detection of individual fluorescent molecules in solution are described, with emphasis on their incorporation into a quantitative Monte Carlo simulation. The simulation is applied to the conditions of a past experiment [Appl. Opt. 34, 3208 (1995)], which utilizes a sheath flow system for high detection efficiency, and it generates comparable results, while helping to identify experimental limitations. The simulation indicates that the use of low dead-time electronics and appropriate selection of experimental parameters should enable detection at more rapid rates for applications in which large numbers of molecules are to be efficiently counted.

© 1998 Optical Society of America

OCIS Codes
(040.1880) Detectors : Detection
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(300.2530) Spectroscopy : Fluorescence, laser-induced

History
Original Manuscript: July 17, 1997
Revised Manuscript: December 18, 1997
Published: April 20, 1998

Citation
Dennis H. Bunfield and Lloyd M. Davis, "Monte Carlo simulation of a single-molecule detection experiment," Appl. Opt. 37, 2315-2326 (1998)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-37-12-2315

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