We describe the quantitative analysis of some metabolic gases bymultichannel Raman spectroscopy. Raman spectra were measured forair, acetone, ammonia, carbon dioxide, and mixed gas consisting ofacetone, ammonia, and air. We designed a new elliptic–sphericalintegration type of cell holder to obtain the Raman spectra of gaseswith a high signal-to-noise ratio. Concentrations of acetone, ammonia, and carbon dioxide were determined by the peak intensities ofRaman bands at 2940, 3228, and 1385 cm−1, respectively. To compensate for the fluctuations of Ramanintensities caused by several factors, such as the fluctuations oflaser power, the peak intensity of a band at 2324 cm−1 dueto nitrogen gas was used as an internal intensity standard. Thecorrelation coefficient between the corrected Raman intensity at 2940cm−1 and the concentration of acetone was calculated to be0.984 for a concentration range of 2–12 ppm. The detection limitof acetone gas was found to be 2 ppm.
© 1998 Optical Society of America
H. Yamamoto, H. Uenoyama, K. Hirai, X. Dou, and Y. Ozaki, "Quantitative Analysis of Metabolic Gases by Multichannel Raman Spectroscopy: Use of a Newly Designed Elliptic-Spherical Integration Type of Cell Holder," Appl. Opt. 37, 2640-2645 (1998)