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Applied Optics

Applied Optics


  • Vol. 37, Iss. 13 — May. 1, 1998
  • pp: 2749–2754

Penetration depth limits of in vivo confocal reflectance imaging

Colin L. Smithpeter, Andrew K. Dunn, A. J. Welch, and Rebecca Richards-Kortum  »View Author Affiliations

Applied Optics, Vol. 37, Issue 13, pp. 2749-2754 (1998)

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We present experiments to predict the maximum penetration depth at which typical biological structures in amelanotic tissue can be detected with confocal microscopy. The detected signal is examined as the signal source strength (index of refraction mismatch), the source depth, and the medium scattering coefficient are varied. The detected background produced by scattering outside the focal volume is examined as the medium scattering coefficient, the depth in the medium, the dimensionless pinhole radius, ν p , and the shape of the scattering phase function are varied. When the system approaches ideal confocal performance (ν p ≃ 3), the penetration depth is limited by the signal-to-noise ratio to approximately 3–4 optical depths (OD’s) for a 0.05 index mismatch. As ν p increases to 8, the penetration depth is limited by the signal-to-background ratio and is dependent on the scattering coefficient. At μ s = 100 cm-1 (l s = 100 μm) and an index mismatch of 0.05, the maximum penetration depth is approximately 2 OD.

© 1998 Optical Society of America

OCIS Codes
(110.0110) Imaging systems : Imaging systems
(120.5700) Instrumentation, measurement, and metrology : Reflection
(290.0290) Scattering : Scattering

Original Manuscript: August 11, 1997
Revised Manuscript: January 16, 1998
Published: May 1, 1998

Colin L. Smithpeter, Andrew K. Dunn, A. J. Welch, and Rebecca Richards-Kortum, "Penetration depth limits of in vivo confocal reflectance imaging," Appl. Opt. 37, 2749-2754 (1998)

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  1. J. V. Jester, P. M. Andrews, W. M. Petroll, M. A. Lamp, H. D. Cavanagh, “In vivo, real-time confocal imaging,” J. Electron Microsc. Tech. 18, 50–60 (1991). [CrossRef] [PubMed]
  2. B. Masters, A. A. Thaer, “Real-time scanning slit confocal microscopy of the in vivo human cornea,” Appl. Opt. 33, 695–701 (1994). [CrossRef] [PubMed]
  3. M. Rajadhyaksha, M. Grossman, D. Esterowitz, R. H. Webb, R. R. Anderson, “In vivo confocal scanning laser microscopy of human skin: melanin provides strong contrast,” J. Invest. Dermatol. 104, 946–952 (1995). [CrossRef] [PubMed]
  4. J. Izatt, M. Hee, G. Owen, “Optical coherence microscopy in scattering media,” Opt. Lett. 19, 590–592 (1994). [CrossRef] [PubMed]
  5. J. Schmitt, A. Knuttel, M. Yadlowsky, “Confocal microscopy in turbid media,” J. Opt. Soc. Am. A 11, 2226–2235 (1994). [CrossRef]
  6. M. Kempe, W. Rudolph, E. Welsch, “Comparative study of confocal and heterodyne microscopy for imaging through scattering media,” J. Opt. Soc. Am. A 13, 46–52 (1996). [CrossRef]
  7. A. K. Dunn, C. Smithpeter, A. Welch, R. Richards-Kortum, “Sources of contrast in confocal reflectance imaging,” Appl. Opt. 35, 3441–3446 (1996). [CrossRef] [PubMed]
  8. H. van de Hulst, Light Scattering by Small Particles (Dover, New York, 1957).
  9. S. Prahl, M. V. Gemert, A. Welch, “Determining the optical properties of turbid media by using the adding–doubling method,” Appl. Opt. 32, 559–568 (1993). [CrossRef] [PubMed]
  10. J. Maier, S. Walker, S. Fantini, M. Franceschini, E. Gratton, “Possible correlation between blood glucose concentration and the reduced scattering coefficient of tissues in the near infrared,” Opt. Lett. 19, 2062–2064 (1994). [CrossRef] [PubMed]
  11. T. Wilson, “The role of the pinhole in confocal imaging systems,” in Handbook of Biological Confocal Microscopy, J. Pawley, ed. (Plenum, New York, 1995), pp. 99–113.
  12. C. J. Sheppard, M. Gu, K. Brain, H. Zhou, “Influence of spherical aberration on axial imaging of confocal reflection microscopy,” Appl. Opt. 33, 616–624 (1994). [CrossRef] [PubMed]
  13. W. Starr, “Light dosimetry in vivo,” Phys. Med. Biol. 42, 763–787 (1997). [CrossRef]
  14. J. Mourant, J. Boyer, A. Hielscher, I. Bigio, “Influence of the scattering phase function on light transport measurements in turbid media performed with small source–detector separations,” Opt. Lett. 21, 546–548 (1996). [CrossRef] [PubMed]
  15. A. K. Dunn, R. Richards-Kortum, “Three-dimensional computation of light scattering from cells,” IEEE J. Sel. Top. Quantum Electron. 2, 898–905 (1996). [CrossRef]

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