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Applied Optics

Applied Optics


  • Vol. 37, Iss. 13 — May. 1, 1998
  • pp: 2766–2773

Multiphoton fluorescence excitation in continuous-wave infrared optical traps

Zhanxiang Zhang, Gregory J. Sonek, Hong Liang, Michael W. Berns, and Bruce J. Tromberg  »View Author Affiliations

Applied Optics, Vol. 37, Issue 13, pp. 2766-2773 (1998)

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The multiphoton fluorescence excitation observed in a continuous-wave (cw) single-beam gradient force optical trap (optical tweezers) is reported for latex beads labeled with a yellow-green fluorescent dye (BODIPY). The fluorescence emission spectra of the yellow-green beads trapped and excited by the same 1064-nm laser light are identical to the spectra excited by the 365-nm UV light. The influence of the numerical aperture of the objective on the slope of the log–log power-dependence has been demonstrated for BODIPY–Oil solution under cw and pulsed-laser conditions. The possibility that three-photon excitation process occurs is discussed within the context of a dog-bone saturation model. Other possibilities for the observed fluorescence excitation have been discussed.

© 1998 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(190.4180) Nonlinear optics : Multiphoton processes

Original Manuscript: August 21, 1997
Revised Manuscript: January 15, 1998
Published: May 1, 1998

Zhanxiang Zhang, Gregory J. Sonek, Hong Liang, Michael W. Berns, and Bruce J. Tromberg, "Multiphoton fluorescence excitation in continuous-wave infrared optical traps," Appl. Opt. 37, 2766-2773 (1998)

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