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Applied Optics

Applied Optics


  • Vol. 37, Iss. 16 — Jun. 1, 1998
  • pp: 3574–3581

Design and optimization of a near-field scanning optical microscope for imaging biological samples in liquid

Levi A. Gheber, Jeeseong Hwang, and Michael Edidin  »View Author Affiliations

Applied Optics, Vol. 37, Issue 16, pp. 3574-3581 (1998)

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We describe a near-field scanning optical microscope capable of imaging biological samples in liquid. The microscope uses a straight optical fiber near-field probe and optical shear-force feedback for tip–sample distance regulation. Physical aspects of the design are discussed, and phenomena related to operation in liquid are revealed. Careful calibration of the instrument in air and in liquid is shown, and for the first time to our knowledge, near-field fluorescence images of a biological cell in liquid are presented.

© 1998 Optical Society of America

OCIS Codes
(170.1420) Medical optics and biotechnology : Biology
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy

Original Manuscript: October 2, 1997
Revised Manuscript: January 16, 1998
Published: June 1, 1998

Levi A. Gheber, Jeeseong Hwang, and Michael Edidin, "Design and optimization of a near-field scanning optical microscope for imaging biological samples in liquid," Appl. Opt. 37, 3574-3581 (1998)

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  1. A. Lewis, K. Lieberman, “Near-field optical imaging with a non-evanescently excited high-brightness light source of sub-wavelength dimensions,” Nature (London) 354, 214–216 (1991). [CrossRef]
  2. E. Betzig, P. L. Finn, J. S. Weiner, “Combined shear force and near-field scanning optical microscopy,” Appl. Phys. Lett. 60, 2484–2486 (1992). [CrossRef]
  3. G. Binning, H. Rohrer, “Scanning tunneling microscopy,” Surf. Sci. 126, 236–244 (1983). [CrossRef]
  4. G. Binning, C. F. Quate, C. Gerber, “Atomic force microscope,” Phys. Rev. Lett. 56, 930–933 (1986). [CrossRef]
  5. H. Muramatsu, N. Chiba, K. Homma, K. Nakajima, T. Ataka, S. Ohta, A. Kusumi, M. Fujihira, “Near-field optical microscopy in liquids,” Appl. Phys. Lett. 66, 3245–3247 (1995). [CrossRef]
  6. R. U. Maheswari, S. Mononobe, H. Tatsumi, Y. Katayama, M. Ohtsu, “Observation of subcellular structures of neurons by an illumination mode near-field optical microscope under an optical feedback control,” Opt. Rev. 3, 463–467 (1996). [CrossRef]
  7. M. Naya, R. Micheletto, S. Mononobe, R. U. Maheswari, M. Ohtsu, “Near-field optical imaging of flagellar filaments of salmonella in water with optical feedback control,” Appl. Opt. 36, 1681–1683 (1997). [CrossRef] [PubMed]
  8. T. H. Keller, T. Rayment, D. Klenerman, R. J. Stephenson, “Scanning near-field optical microscopy in reflection mode imaging in liquid,” Rev. Sci. Instrum. 68, 1448–1454 (1997). [CrossRef]
  9. P. J. Moyer, S. B. Kammer, “High-resolution imaging using near-field scanning optical microscopy and shear force feedback in water,” Appl. Phys. Lett. 68, 3380–3382 (1996). [CrossRef]
  10. M. Mertesdorf, M. Schonhoff, F. Lohr, S. Kirstein, “Scanning near-field optical microscope designed for operation in liquids,” Surf. Interface Anal. 25, 755–759 (1997). [CrossRef]
  11. PZT-5H piezo tube from Staveley Sensors, Inc., 91 Prestige Park Circle, East Hartford, Conn. 08108-5420, telephone (860) 289-5428.
  12. InGaAlP T9225 diode laser, Toshiba Corporation, 1-1-1. Shibaura Minatoku, Tokyo 105-01, Japan.
  13. FS-SN-3224 single-mode optical fiber, 3M, 420 Frontage Road, West Haven, Conn. 06516-4190, telephone (230) 934-7961.
  14. PAC022 Achromatic lens, Newport Corporation, 1791 Deere Avenue, Irvine, Calif. 92714, telephone (800) 222-6440.
  15. J. W. P. Hsu, M. Lee, B. S. Deaver, “A nonoptical tip-sample distance control method for near-field scanning optical microscopy using impedance changes in an electromechanical system,” Rev. Sci. Instrum. 66, 3177–3181 (1995). [CrossRef]
  16. K. Karrai, R. Grober, “Piezoelectric tip-sample distance control for near-field optical microscopes,” Appl. Phys. Lett. 66, 1842–1843 (1995). [CrossRef]
  17. 0.310-in. (0.079-cm)-diameter, 0.010-in. (0.025-cm)-thick quartz window, ESCO Products, Inc., 171 Oak Ridge Road, Oak Ridge, N.J. 07438, telephone (800) 922-3726.
  18. Type 316W stainless-steel hypodermic tubing, Small Parts Inc., 13980 N.W. 58th Court, Miami Lakes, Fla. 33014-0650, telephone (800) 220-4242.
  19. High-speed silicon p-i-n photodiode, Model 13DSI 007, Melles Griot, 1770 Kettering Street, Irvine, Calif. 92714, telephone (800) 835-2626.
  20. Wide bandwidth amplifier, Model 13 AMP 005, Melles Griot, 1770 Kettering Street, Irvine, Calif. 92714, telephone (800) 835-2626.
  21. Lock-in amplifier, Model 5210, EG&G Princeton Applied Research, P.O. Box 2565, Princeton, N.J. 08543-2565, telephone (609) 530-1000.
  22. STM 100, RHK Technology, Inc., 1750 West Hamlin Road., Rochester Hills, Mich. 48309, telephone (810) 656-3116.
  23. Axiovert 100 microscope, Zeiss, One Zeiss Drive, Thornwood, N.Y. 10594, telephone (800) 233-2343.
  24. Achrostigmat objective, Model 440280, Zeiss, One Zeiss Drive, Thornwood, N.Y. 10594, telephone (800) 233-2343.
  25. Single-photon-counting module, Model SPCM-200 CD1718, EG&G Optoelectronics Canada, 221 Commerce Drive, Montgomeryville, Pa. 18936, telephone (514) 424-3361.
  26. Omega Optical, Inc., P.O. Box 573, 3 Grove Street, Brattleboro, Vt. 05302-0573.
  27. M. J. Gregor, P. G. Blome, J. Schöfer, R. G. Ulbrich, “Probe-surface interaction in near-field optical microscopy: the nonlinear bending force mechanism,” Appl. Phys. Lett. 68, 307–309 (1996). [CrossRef]
  28. Handbook of Chemistry and Physics, 48th ed. (Chemical Rubber, Cleveland, Ohio, 1967–1968).
  29. Dubbel Handbook of Mechanical Engineering (Springer-Verlag, Heidelberg, Germany, 1995).
  30. Standard Handbook for Mechanical Engineers, 7th ed. (McGraw-Hill, New York, 1967).
  31. 1-μm pitch calibration grating, Park Scientific Instruments, 1171 Borregas Avenue, Sunnyvale, Calif. 94089, telephone (408) 747-1600.

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