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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 37, Iss. 16 — Jun. 1, 1998
  • pp: 3574–3581

Design and Optimization of a Near-Field Scanning Optical Microscope for Imaging Biological Samples in Liquid

Levi A. Gheber, Jeeseong Hwang, and Michael Edidin  »View Author Affiliations


Applied Optics, Vol. 37, Issue 16, pp. 3574-3581 (1998)
http://dx.doi.org/10.1364/AO.37.003574


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Abstract

We describe a near-field scanning optical microscope capable of imaging biological samples in liquid. The microscope uses a straight optical fiber near-field probe and optical shear-force feedback for tip–sample distance regulation. Physical aspects of the design are discussed, and phenomena related to operation in liquid are revealed. Careful calibration of the instrument in air and in liquid is shown, and for the first time to our knowledge, near-field fluorescence images of a biological cell in liquid are presented.

© 1998 Optical Society of America

OCIS Codes
(170.1420) Medical optics and biotechnology : Biology
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy

Citation
Levi A. Gheber, Jeeseong Hwang, and Michael Edidin, "Design and Optimization of a Near-Field Scanning Optical Microscope for Imaging Biological Samples in Liquid," Appl. Opt. 37, 3574-3581 (1998)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-37-16-3574


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