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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 37, Iss. 22 — Aug. 1, 1998
  • pp: 5344–5347

Absolute emission spectra from Bacillus subtilis and Escherichia coli vegetative cells in solution

Mark Seaver, Dominick C. Roselle, Joseph F. Pinto, and Jay D. Eversole  »View Author Affiliations


Applied Optics, Vol. 37, Issue 22, pp. 5344-5347 (1998)
http://dx.doi.org/10.1364/AO.37.005344


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Abstract

Spectrally resolved emission (270–560 nm) from dilute suspensions of washed Bacillus subtilis and Escherichia coli were measured by use of tunable laser excitation between 270 and 300 nm. Integrated absolute emission cross sections increase with decreasing excitation wavelength and range from 1.8 × 10-12 to 6.0 × 10-11 cm2/(particle sr). An emission band near 340 nm dominates all observed spectra. At each excitation wavelength spectrally resolved emissions from the E. coli and B. subtilis suspensions are indistinguishable.

© 1998 Optical Society of America

OCIS Codes
(170.1420) Medical optics and biotechnology : Biology
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(300.2530) Spectroscopy : Fluorescence, laser-induced

History
Original Manuscript: January 7, 1998
Revised Manuscript: April 9, 1998
Published: August 1, 1998

Citation
Mark Seaver, Dominick C. Roselle, Joseph F. Pinto, and Jay D. Eversole, "Absolute emission spectra from Bacillus subtilis and Escherichia coli vegetative cells in solution," Appl. Opt. 37, 5344-5347 (1998)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-37-22-5344

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