OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 37, Iss. 22 — Aug. 1, 1998
  • pp: 5344–5347

Absolute emission spectra from Bacillus subtilis and Escherichia coli vegetative cells in solution

Mark Seaver, Dominick C. Roselle, Joseph F. Pinto, and Jay D. Eversole  »View Author Affiliations


Applied Optics, Vol. 37, Issue 22, pp. 5344-5347 (1998)
http://dx.doi.org/10.1364/AO.37.005344


View Full Text Article

Enhanced HTML    Acrobat PDF (81 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Spectrally resolved emission (270–560 nm) from dilute suspensions of washed Bacillus subtilis and Escherichia coli were measured by use of tunable laser excitation between 270 and 300 nm. Integrated absolute emission cross sections increase with decreasing excitation wavelength and range from 1.8 × 10-12 to 6.0 × 10-11 cm2/(particle sr). An emission band near 340 nm dominates all observed spectra. At each excitation wavelength spectrally resolved emissions from the E. coli and B. subtilis suspensions are indistinguishable.

© 1998 Optical Society of America

OCIS Codes
(170.1420) Medical optics and biotechnology : Biology
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(300.2530) Spectroscopy : Fluorescence, laser-induced

History
Original Manuscript: January 7, 1998
Revised Manuscript: April 9, 1998
Published: August 1, 1998

Citation
Mark Seaver, Dominick C. Roselle, Joseph F. Pinto, and Jay D. Eversole, "Absolute emission spectra from Bacillus subtilis and Escherichia coli vegetative cells in solution," Appl. Opt. 37, 5344-5347 (1998)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-37-22-5344


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. C. R. Cantor, P. R. Schimmel, Biophysical Chemistry (W. H. Freeman, San Francisco, 1980), pp. 380, 443.
  2. S. V. Konev, Fluorescence and Phosphorescence of Proteins and Nucleic Acids (Plenum, New York, 1967), p. 10.
  3. T. D. Brock, M. T. Madigan, J. M. Martinko, J. Parker, Biology of Microorganisms, 7th ed. (Prentice Hall, Englewood Cliffs, N.J., 1994) Chap. 19.
  4. D. C. Shelly, J. M. Quarles, I. M. Warner, “Identification of fluorescent Pseudomonas species,” Clin. Chem. 26, 1127–1132 (1980). [PubMed]
  5. D. C. Shelly, J. M. Quarles, I. M. Warner, “Multiparameter approach to the ‘fingerprint’ of fluorescent Pseudomonas,” Clin. Chem. 26, 1419–1424 (1980). [PubMed]
  6. M. J. Sorrell, J. Tribble, L. Reinisch, J. A. Werkhaven, R. H. Ossoff, “Bacteria identification of otitis media with fluorescence spectroscopy,” Lasers Surg. Med. 14, 155–163 (1994). [CrossRef] [PubMed]
  7. R. A. Dalterio, W. H. Nelson, D. Britt, J. Sperry, D. Psaras, J. F. Tanquay, S. L. Suib, “Steady-state and decay characteristics of protein tryptophan fluorescence from bacteria,” Appl. Spectrosc. 40, 86–90 (1986). [CrossRef]
  8. R. A. Dalterio, W. H. Nelson, D. Britt, J. Sperry, J. F. Tanquay, S. L. Suib, “Steady-state and decay characteristics of primary fluorescence from live bacteria,” Appl. Spectrosc. 41, 234–241 (1987). [CrossRef]
  9. B. V. Bronk, L. Reinisch, “Variability of steady-state bacterial fluorescence with respect to growth conditions,” Appl. Spectrosc. 47, 436–440 (1991). [CrossRef]
  10. G. W. Faris, R. A. Copeland, K. Mortelmans, B. V. Bronk, “Spectrally resolved absolute fluorescence cross sections for Bacillus spores,” Appl. Opt. 36, 958–967 (1997). [CrossRef] [PubMed]
  11. American Type Culture Collection, 12301 Parklawn Drive, Rockville, Md. 20852-1776.
  12. W. B. Bowden, “Comparison of two direct-count techniques for enumerating aquatic bacteria,” Appl. Environ. Microbiol. 33, 1229–1232 (1977). [PubMed]
  13. D. C. Roselle, M. Seaver, J. D. Eversole, “Changes in intrinsic fluorescence during the production of viable but nonculturable Escherichia coli,” J. Ind. Microbiol. 20 (in press).
  14. J. F. Pinto, L. Esterowitz, G. H. Rosenblatt, “Frequency tripling of a Q-switched Cr:LiSAF laser to the UV region,” IEEE J. Sel. Top. Quantum Electron. 1, 58–63 (1995). [CrossRef]
  15. D. Mauzerall, “Multiple excitation in photosynthetic systems,” Biophys. J. 16, 87–92 (1976). [CrossRef] [PubMed]
  16. B. R. Marshal, R. C. Smith, “Raman scattering and in-water ocean optical properties,” Appl. Opt. 29, 71–84 (1990). [CrossRef]
  17. N. P. Romanov, V. S. Shuklin, “Raman scattering cross section of liquid water,” Opt. Spectrosc. 38, 646–648 (1970).
  18. I. I. Kondelenko, P. A. Korotkov, V. A. Klimenko, O. P. Demyanenko, “Transverse cross section of the Raman scattering of the n1 vibration of the water molecule in the liquid and gaseous states,” Opt. Spectrosc. 43, 384–386 (1975).
  19. R. B. Slusher, V. E. Derr, “Temperature dependence and cross sections of some Stokes and anti-Stokes Raman lines in ice Ih,” Appl. Opt. 14, 2116–2120 (1975). [CrossRef] [PubMed]
  20. C. H. Chang, L. A. Young, “Seawater temperature measurements from Raman spectra,” Res. Note 920, N62269-72-C-0204, ARPA Order No. 1911 (July1972).
  21. S. Sugihara, M. Kishino, M. J. Okami, “Contribution of Raman scattering to upward irradiance in the sea,” J. Oceanogr. Soc. Jpn. 40, 397–404 (1984). [CrossRef]
  22. G. W. Faris, R. A. Copeland, “Wavelength dependence of the Raman cross section for liquid water,” Appl. Opt. 36, 2686–2688 (1997). [CrossRef] [PubMed]
  23. S. D. Christesen, K. K. Ong, “Fluorescence spectroscopy of biological agents: I. Brucella melitensis, Brucella abortus and Brucella suis,” ERDEC Internal Rep., contact S. Christesen, Attn.: SCBRD-RTE, U.S. Army Edgewood RDEC, Aberdeen Proving Ground, Md. 21010-5423.
  24. Sandia National Laboratory, “UV detection of biological species,” ERDEC Internal Rep., contact S. Christesen, Attn.: SCBRD-RTE, U.S. Army Edgewood RDEC, Aberdeen Proving Ground, Md. 21010-5423.

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 

« Previous Article

OSA is a member of CrossRef.

CrossCheck Deposited