The photosensitizer hematoporphyrin (HP) was site specifically attached to a murine monoclonal antibody (MAb) fragment OC125F(ab′)<sub>2</sub> directed against ovarian cancer cells and to nonspecific rabbit immunoglobulin G. The photoimmunoconjugates were positively charged and were purified by column chromatography. The OC125F(ab′)<sub>2</sub> conjugate retained immunoreactivity with human ovarian cancer cells, and the binding was competed with unmodified MAb. Phototoxicity paralleled the cellular uptake with the OC125F(ab′)<sub>2</sub> conjugate and the light showing selective killing of target cells compared with nontarget cells. Nontargeted conjugates and free HP produced lower levels of phototoxicity and showed no selectivity.
© 1998 Optical Society of America
(170.5180) Medical optics and biotechnology : Photodynamic therapy
Michael R. Hamblin, Michael P. Bamberg, Jaimie L. Miller, and Tayyaba Hasan, "Cationic Photoimmunoconjugates Between Monoclonal Antibodies and Hematoporphyrin: Selective Photodestruction of Ovarian Cancer Cells," Appl. Opt. 37, 7184-7192 (1998)