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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 37, Iss. 31 — Nov. 1, 1998
  • pp: 7378–7391

Thermal modeling of Laser Capture Microdissection

Seth R. Goldstein, Philip G. McQueen, and Robert F. Bonner  »View Author Affiliations


Applied Optics, Vol. 37, Issue 31, pp. 7378-7391 (1998)
http://dx.doi.org/10.1364/AO.37.007378


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Abstract

A first-order thermal analysis is applied to Laser Capture Microdissection (LCM), a new microscope technique for routine targeting and extraction of specific cells from tissue sections for subsequent multiplex molecular analysis. In LCM a polymer film placed in contact with the tissue is focally activated by a pulsed IR laser beam and is melted and bonded to adjacent targeted cells. A three-dimensional finite-element model is used to predict the thermal transients within the polymer, the captured tissue, and its macromolecules. The simulations allow a comparison of models for the physical process of LCM with the experimental data on the dependence of the transfer spot size on laser power. The validated physical model and the thermal simulations permit optimization of the complex LCM parameter space for a wide variety of configurations and applications.

© 1998 Optical Society of America

OCIS Codes
(140.6810) Lasers and laser optics : Thermal effects
(160.6840) Materials : Thermo-optical materials
(170.1530) Medical optics and biotechnology : Cell analysis
(170.4730) Medical optics and biotechnology : Optical pathology
(350.5340) Other areas of optics : Photothermal effects

History
Original Manuscript: March 5, 1998
Revised Manuscript: July 6, 1998
Published: November 1, 1998

Citation
Seth R. Goldstein, Philip G. McQueen, and Robert F. Bonner, "Thermal modeling of Laser Capture Microdissection," Appl. Opt. 37, 7378-7391 (1998)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-37-31-7378


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References

  1. M. R. Emmert-Buck, R. F. Bonner, P. D. Smith, R. F. Chuaqui, Z. Zhuang, S. R. Goldstein, R. A. Weiss, L. A. Liotta, “Laser capture microdissection,” Science 274, 998–1001 (1996). [CrossRef] [PubMed]
  2. R. F. Bonner, M. R. Emmert-Buck, K. Cole, T. Pohida, R. F. Chuaqui, S. R. Goldstein, L. A. LiottaTech. Sight, “Laser capture microdissection: molecular analysis of tissue,” Science 278, 1481–1483 (1997).
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  8. A. Kirk, Packaging and Industrial Polymers Division, DuPont, Wilmington, Del. 19880 (personal communication, 1997).
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  10. Ref. 8, p. 264.
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