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Applied Optics

Applied Optics


  • Vol. 37, Iss. 31 — Nov. 1, 1998
  • pp: 7410–7418

Single scattering by red blood cells

Martin Hammer, Dietrich Schweitzer, Bernhard Michel, Eike Thamm, and Achim Kolb  »View Author Affiliations

Applied Optics, Vol. 37, Issue 31, pp. 7410-7418 (1998)

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A highly diluted suspension of red blood cells (hematocrit 0.01) was illuminated with an Ar or a dye laser in the wavelength range of 458–660 nm. The extinction and the angle-resolved intensity of scattered light were measured and compared with the predictions of Mie theory, the Rayleigh–Gans approximation, and the anomalous diffraction approximation. Furthermore, empirical phase functions were fitted to the measurements. The measurements were in satisfactory agreement with the predictions of Mie theory. However, better agreement was found with the anomalous diffraction model. In the Rayleigh–Gans approximation, only small-angle scattering is described appropriately. The scattering phase function of erythrocytes may be represented by the Gegenbauer kernel phase function.

© 1998 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.1530) Medical optics and biotechnology : Cell analysis
(170.7050) Medical optics and biotechnology : Turbid media
(290.0290) Scattering : Scattering
(290.5820) Scattering : Scattering measurements

Original Manuscript: March 11, 1998
Revised Manuscript: June 22, 1998
Published: November 1, 1998

Martin Hammer, Dietrich Schweitzer, Bernhard Michel, Eike Thamm, and Achim Kolb, "Single scattering by red blood cells," Appl. Opt. 37, 7410-7418 (1998)

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