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Applied Optics

Applied Optics


  • Vol. 37, Iss. 34 — Dec. 1, 1998
  • pp: 8112–8119

High-speed spectral imager for imaging transient fluorescence phenomena

Curtis E. Volin, Bridget K. Ford, Michael R. Descour, John P. Garcia, Daniel W. Wilson, Paul D. Maker, and Gregory H. Bearman  »View Author Affiliations

Applied Optics, Vol. 37, Issue 34, pp. 8112-8119 (1998)

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We describe fluorescence spectral imaging results with the microscope computed-tomography imaging spectrometer (μCTIS). This imaging spectrometer is capable of recording spatial and spectral data simultaneously. Consequently, μCTIS can be used to image dynamic phenomena. The results presented consist of proof-of-concept imaging results with static targets composed of 6-μm fluorescing microspheres. Image data were collected with integration times of 16 ms, comparable with video-frame-rate integration times. Conversion of raw data acquired by the μCTIS to spatial and spectral data requires postprocessing. The emission spectra were sampled at 10-nm intervals between 420 and 710 nm. The smallest spatial sampling interval presented is 1.7 μm.

© 1998 Optical Society of America

OCIS Codes
(000.1430) General : Biology and medicine
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(300.6500) Spectroscopy : Spectroscopy, time-resolved

Original Manuscript: April 27, 1998
Revised Manuscript: September 8, 1998
Published: December 1, 1998

Curtis E. Volin, Bridget K. Ford, Michael R. Descour, John P. Garcia, Daniel W. Wilson, Paul D. Maker, and Gregory H. Bearman, "High-speed spectral imager for imaging transient fluorescence phenomena," Appl. Opt. 37, 8112-8119 (1998)

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  1. I. Newton, “Prop. V, Theorem IV,” Opticks (Dover, New York, 1952), p. 1704.
  2. Y. Garini, N. Katzir, D. Cabib, R. A. Buckwald, D. G. Soenksen, S. Malik, “Spectral bio-imaging,” Fluorescence Imaging Spectrometry and Microscopy, X. F. Wang, B. Herman, eds. (Wiley, New York, 1996), pp. 87–124.
  3. J. M. Mooney, V. E. Vickers, A. Myoung, A. K. Brodzik, “High-throughput hyperspectral infrared camera,” J. Opt. Soc. Am. A 14, 2951–2961 (1997). [CrossRef]
  4. E. S. Wachman, W. H. Niu, D. L. Farkas, “Imaging acousto-optic tunable filter with 0.35-micrometer spatial resolution,” Appl. Opt. 35, 5220–5226 (1996). [CrossRef] [PubMed]
  5. C. Hoyt, “Liquid crystal tunable filters clear the way for imaging multiprobe fluorescence,” Biophotonics International 4, 49–51 (1996).
  6. M. R. Descour, C. E. Volin, T. M. Gleeson, E. L. Dereniak, M. F. Hopkins, D. W. Wilson, P. D. Maker, “Demonstration of a computed-tomography imaging spectrometer using a computer-generated hologram disperser,” Appl. Opt. 36, 3694–3698 (1997). [CrossRef] [PubMed]
  7. X. F. Wang, B. Herman, Fluorescence Imaging Spectrometry and Microscopy (Wiley, New York, 1996).
  8. R. Y. Tsien, B. J. Bacskai, “Video-rate confocal microscopy,” Handbook of Biological Confocal Microscopy (Plenum, New York, 1995), pp. 459–478. [CrossRef]
  9. R. Martínez-Zaguilán, M. W. Gurulé, R. M. Lynch, “Simultaneous measurement of intracellular pH and Ca2+ in insulin-secreting cells by spectral imaging microscopy,” Am. J. Physiol. Cell Physiol. 270C1438–C1446 (1996).
  10. G. A. Wagnières, A. P. Studzinski, D. R. Braichotte, P. Monnier, C. Despeursinge, A. Châtelain, H. E. van den Bergh, “Clinical imaging fluorescence apparatus for the endoscopic photodetection of early cancers by use of Photofrin II,” Appl. Opt. 36, 5608–5620 (1997). [CrossRef] [PubMed]
  11. R. Y. Tsien, A. Waggoner, “Fluorophores for confocal microscopy,” Handbook of Biological Confocal Microscopy (Plenum, New York, 1995) pp. 267–279. [CrossRef]
  12. D. R. Sandison, R. M. Williams, K. S. Wells, J. Strickler, W. W. Webb, “Quantitative fluorescence confocal laser scanning microscopy (CLSM),” Handbook of Biological Confocal Microscopy (Plenum, New York, 1995), pp. 39–53. [CrossRef]
  13. M. R. Descour, C. E. Volin, E. L. Dereniak, K. J. Thome, A. B. Schumacher, D. W. Wilson, P. D. Maker, “Demonstration of a high-speed nonscanning imaging spectrometer,” Opt. Lett. 22, 1271–1273 (1997). [CrossRef] [PubMed]
  14. W. H. Press, S. Teukolsky, W. Vetterling, B. Flannery, Numerical Recipes in C pp. 78–81 (Cambridge U Press, N.Y., 1992).
  15. A. Lent, “A convergent algorithm for maximum entropy image restoration,” in Image Analysis and Evaluation, SPSE Conference Proceedings, R. Shaw, ed. (Society of Photographic Scientists and Engineers, n.p.), pp. 249–257.
  16. Fluoresbrite polychromatic microspheres (6 μm), Catalog no. 19111, Polysciences Inc., 400 Valley Road, Warrington, Pa. 18976.
  17. S2000 Fiber Spectrometer, Ocean Optics, Inc., 380 Main Street, Dunedin, Fla. 34698.
  18. Optronic Laboratories, 4632 36th Street, Orlando, Fla. 32811; 1000-W Spectral Irradiance Standard, courtesy of Stuart Biggar, University of Arizona Optical Sciences Center, Remote Sensing Group (1998).
  19. Nikon, USA, 1300 Walt Whitman Road, Melville, N.Y. 11747-3064.

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