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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 37, Iss. 34 — Dec. 1, 1998
  • pp: 8112–8119

High-speed spectral imager for imaging transient fluorescence phenomena

Curtis E. Volin, Bridget K. Ford, Michael R. Descour, John P. Garcia, Daniel W. Wilson, Paul D. Maker, and Gregory H. Bearman  »View Author Affiliations


Applied Optics, Vol. 37, Issue 34, pp. 8112-8119 (1998)
http://dx.doi.org/10.1364/AO.37.008112


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Abstract

We describe fluorescence spectral imaging results with the microscope computed-tomography imaging spectrometer (μCTIS). This imaging spectrometer is capable of recording spatial and spectral data simultaneously. Consequently, μCTIS can be used to image dynamic phenomena. The results presented consist of proof-of-concept imaging results with static targets composed of 6-μm fluorescing microspheres. Image data were collected with integration times of 16 ms, comparable with video-frame-rate integration times. Conversion of raw data acquired by the μCTIS to spatial and spectral data requires postprocessing. The emission spectra were sampled at 10-nm intervals between 420 and 710 nm. The smallest spatial sampling interval presented is 1.7 μm.

© 1998 Optical Society of America

OCIS Codes
(000.1430) General : Biology and medicine
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(300.6500) Spectroscopy : Spectroscopy, time-resolved

History
Original Manuscript: April 27, 1998
Revised Manuscript: September 8, 1998
Published: December 1, 1998

Citation
Curtis E. Volin, Bridget K. Ford, Michael R. Descour, John P. Garcia, Daniel W. Wilson, Paul D. Maker, and Gregory H. Bearman, "High-speed spectral imager for imaging transient fluorescence phenomena," Appl. Opt. 37, 8112-8119 (1998)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-37-34-8112

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