A method for fluorescence polarization difference imaging is demonstrated for enhancing the image quality of a luminous object embedded in a random medium. The polarization preservation of light propagating in the scattering medium leads to partially polarized light emission by a contrast-agent dye located inside the object. Subtraction of the images of the luminous object detected at two orthogonal polarization directions improves the image resolution compared with a conventional optical imaging approach.
© 1998 Optical Society of America
Original Manuscript: May 5, 1997
Revised Manuscript: August 22, 1997
Published: February 1, 1998
S. G. Demos, W. B. Wang, and R. R. Alfano, "Imaging objects hidden in scattering media with fluorescence polarization preservation of contrast agents," Appl. Opt. 37, 792-797 (1998)