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Applied Optics

Applied Optics


  • Vol. 37, Iss. 4 — Feb. 1, 1998
  • pp: 792–797

Imaging objects hidden in scattering media with fluorescence polarization preservation of contrast agents

S. G. Demos, W. B. Wang, and R. R. Alfano  »View Author Affiliations

Applied Optics, Vol. 37, Issue 4, pp. 792-797 (1998)

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A method for fluorescence polarization difference imaging is demonstrated for enhancing the image quality of a luminous object embedded in a random medium. The polarization preservation of light propagating in the scattering medium leads to partially polarized light emission by a contrast-agent dye located inside the object. Subtraction of the images of the luminous object detected at two orthogonal polarization directions improves the image resolution compared with a conventional optical imaging approach.

© 1998 Optical Society of America

OCIS Codes
(260.2510) Physical optics : Fluorescence
(260.5430) Physical optics : Polarization
(290.0290) Scattering : Scattering

Original Manuscript: May 5, 1997
Revised Manuscript: August 22, 1997
Published: February 1, 1998

S. G. Demos, W. B. Wang, and R. R. Alfano, "Imaging objects hidden in scattering media with fluorescence polarization preservation of contrast agents," Appl. Opt. 37, 792-797 (1998)

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  1. P. S. Anderson, S. Montan, S. Svanberg, “Multicolor imaging system for tissue fluorescence diagnostics; use for tumor and atherosclerotic plaque demarcation,” IEEE J. Quantum Electronics QE-23, 1798–1805 (1987).
  2. K. M. Yoo, Z.-W. Zang, S. A. Ahmed, R. R. Alfano, “Imaging object hidden in scattering media using a fluorescence-absorption technique,” Opt. Lett. 16, 1252–1254 (1991). [CrossRef] [PubMed]
  3. K. Dowling, S. C. W. Hyde, J. C. Dainty, P. M. W. French, “2-D fluorescence lifetime imaging using a time-gated image intensifier,” in Advances in Optical Imaging and Photon Migration, R. R. Alfano, ed., Vol. 2 of OSA Proceedings Series (Optical Society of America, Washington, D.C., 1996), pp. 332–335.
  4. M. A. O’Leary, D. A. Boas, X. D. Li, B. Chance, A. G. Yodh, “Fluorescence lifetime imaging in turbid media,” Opt. Lett. 21, 158–160 (1996). [CrossRef] [PubMed]
  5. K. M. Yoo, F. Liu, R. R. Alfano, “Imaging object hidden in highly scattering media using femtosecond second-harmonic generation cross-correlation time gating,” Opt. Lett. 16, 1019–1021 (1991). [CrossRef] [PubMed]
  6. H. Chen, Y. Chen, D. Dilworth, E. Leith, J. Lopez, J. Valdmanis, “Two-dimensional imaging through diffusion media using 150-fs gated electronic holography techniques,” Opt. Lett. 16, 487–489 (1991). [CrossRef] [PubMed]
  7. L. Wang, P. P. Ho, C. H. Liu, G. Zhang, R. R. Alfano, “Ballistic 2-D imaging through scattering wall using an ultrafast Kerr gate,” Science 253, 769–771 (1991). [CrossRef] [PubMed]
  8. J. M. Schmitt, A. H. Gandjbakhche, R. F. Bonner, “Use of polarized light to discriminate short-path photons in a multiply scattering medium,” Appl. Opt. 31, 6535–6546 (1992). [CrossRef] [PubMed]
  9. S. G. Demos, R. R. Alfano, “Temporal gating in highly scattering media by the degree of optical polarization,” Opt. Lett. 21, 161–163 (1996). [CrossRef] [PubMed]
  10. S. G. Demos, H. Savage, A. S. Heerdt, S. Schantz, R. R. Alfano, “Time-resolved degree of polarization for human breast tissue,” Opt. Commun. 124, 439–442 (1996). [CrossRef]
  11. G. R. Fleming, J. M. Morris, G. W. Robinson, “Direct observation of rotational diffusion by picosecond spectroscopy,” Chem. Phys. 17, 91–100 (1976). [CrossRef]
  12. G. Porter, P. J. Sadkowski, C. J. Tredwell, “Picosecond rotational diffusion in kinetic and steady state fluorescence spectroscopy,” Chem. Phys. Lett. 49, 416–420 (1977). [CrossRef]
  13. W. B. Wang, S. G. Demos, J. Ali, R. R. Alfano, “Imaging fluorescent objects embedded inside animal tissues using polarization difference technique,” Opt. Commun. 142, 161–166 (1997). [CrossRef]

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