Abstract
A novel refractive-index detection scheme used for capillary electrophoresis detection, based on the interference of two retroreflected beams from the outer surface of a capillary tube illuminated by a focused laser beam, is described. A theoretical description and experimental measurement of the intensity profile of the interference fringe pattern in the detection plane are presented. The factors that limit the sensitivity of the proposed refractive-index detection scheme are discussed and compared with the transmitted beam interference and backscatter-based refractive-index detection configurations. The proposed refractive-index detector was used successfully for detection of capillary electrophoresis separations of saccharose, maltose, and lactose with a capillary tube of 50-μm inner diameter and a simple experimental setup.
© 1998 Optical Society of America
Full Article | PDF ArticleMore Like This
Beat Krattiger, Alfredo E. Bruno, H. Michael Widmer, Martial Geiser, and René Dändliker
Appl. Opt. 32(6) 956-965 (1993)
Darryl J. Bornhop
Appl. Opt. 34(18) 3234-3239 (1995)
Shengwen Qi, Xiuqin Yang, Chunping Zhang, Lianshun Zhang, Xinyu Wang, Tang Xu, Jianguo Tian, and Guangyin Zhang
Appl. Opt. 43(3) 530-536 (2004)