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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 37, Iss. 6 — Feb. 20, 1998
  • pp: 998–1005

On-Column Refractive-Index Detection Based on Retroreflected Beam Interference for Capillary Electrophoresis

Yanzhuo Deng and Bincheng Li  »View Author Affiliations


Applied Optics, Vol. 37, Issue 6, pp. 998-1005 (1998)
http://dx.doi.org/10.1364/AO.37.000998


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Abstract

A novel refractive-index detection scheme used for capillary electrophoresis detection, based on the interference of two retroreflected beams from the outer surface of a capillary tube illuminated by a focused laser beam, is described. A theoretical description and experimental measurement of the intensity profile of the interference fringe pattern in the detection plane are presented. The factors that limit the sensitivity of the proposed refractive-index detection scheme are discussed and compared with the transmitted beam interference and backscatter-based refractive-index detection configurations. The proposed refractive-index detector was used successfully for detection of capillary electrophoresis separations of saccharose, maltose, and lactose with a capillary tube of 50-μm inner diameter and a simple experimental setup.

© 1998 Optical Society of America

OCIS Codes
(040.1880) Detectors : Detection
(120.5710) Instrumentation, measurement, and metrology : Refraction
(260.3160) Physical optics : Interference

Citation
Yanzhuo Deng and Bincheng Li, "On-Column Refractive-Index Detection Based on Retroreflected Beam Interference for Capillary Electrophoresis," Appl. Opt. 37, 998-1005 (1998)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-37-6-998


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References

  1. Y.-F. Cheng, S. Wu, D.-Y. Chen, and N. J. Dovichi, “Interaction of capillary zone electrophoresis with a sheath flow cuvette detector,” Anal. Chem. 62, 496–503 (1990).
  2. F. Foret, S. Fanali, L. Ossicini, and P. Bocek, “Indirect photometric detection in capillary zone electrophoresis,” J. Chromatogr. 470, 299–308 (1989).
  3. W. G. Kuhr and E. S. Yeung, “Indirect fluorescence detection of native amino acids in capillary zone electrophoresis,” Anal. Chem. 60, 1832–1834 (1988).
  4. D. J. Bornhop and N. J. Dovichi, “Simultaneous laser-based refractive index and absorbance determinations within microliter diameter capillary tubes,” Anal. Chem. 59, 1632–1636 (1987).
  5. X. Huang, T.-K. Pang, M. J. Gordon, and R. N. Zare, “On-column conductivity detector for capillary zone electrophoresis,” Anal. Chem. 59, 2747–2749 (1987).
  6. D. J. Bornhop and N. J. Dovichi, “Simple nanoliter refractive index detector,” Anal. Chem. 58, 504–505 (1986).
  7. B. Krattiger, A. E. Bruno, H. M. Widmer, M. Geisser, and R. Dandliker, “Laser-based refractive-index detection for capillary electrophoresis: ray-tracing interference theory,” Appl. Opt. 32, 956–965 (1993).
  8. D. J. Bornhop, T. G. Nolan, and N. J. Dovichi, “Subnanoliter laser-based refractive index detector for 0.25 mm I.D. microbore liquid chromatography, reverse-phase separation of nanogram amounts of sugars,” J. Chromatogr. 384, 181–187 (1987).
  9. A. E. Bruno, B. Krattiger, F. Maystre, and H. M. Widmer, “On-column laser-based refractive index detector for capillary electrophoresis,” Anal. Chem. 63, 2689–2697 (1991).
  10. B. Krattiger, G. J. M. Bruln, and A. E. Bruno, “Hologram-based refractive index detector for capillary electrophoresis: separation of metal ions,” Anal. Chem. 66, 1–8 (1994).
  11. M. Yu and N. J. Dovichi, “Sub-femtomole determination of DABSYL-amino acids using capillary zone electrophoresis and laser-based thermo-optical absorbance detection,” Mikrochim. Acta 3, 27–40 (1988).
  12. M. Yu and N. J. Dovichi, “Attomole amino acid analysis by capillary zone electrophoresis with thermo-optical absorbance detection,” Anal. Chem. 61, 37–40 (1989).
  13. B. Krattiger, A. E. Bruno, H. M. Widmer, and R. Dandliker, “Hologram-based thermooptical absorbance detection in capillary electrophoresis: separation of nucleosides and nucleotides,” Anal. Chem. 67, 124–130 (1995).
  14. J. M. Saz, B. Krattiger, A. E. Bruno, J. C. Diez-Masa, and H. M. J. Widmer, “Thermo-optical absorbance detection of native proteins separated by capillary electrophoresis in 10 μm I.D. tubes,” J. Chromatogr. A 699, 315–322 (1995).
  15. D. J. Bornhop, “Microvolume index of refraction determinations by interferometric backscatter,” Appl. Opt. 34, 3234–3239 (1995).
  16. H. J. Tarigan, P. Neill, C. K. Kenmore, and D. J. Bornhop, “Capillary-scale refractive index detection by interferometric backscatter,” Anal. Chem. 68, 1762–1770 (1996).
  17. T. Tsuda, J. V. Sweedler, and R. N. Zare, “Rectangular capillaries for capillary zone electrophoresis,” Anal. Chem. 62, 2149–2152 (1990).
  18. J. W. Jorgenson and K. D. Lukacs, “Zone electrophoresis in open-tubular glass capillaries,” Anal. Chem. 53, 1298–1302 (1981).
  19. B. C. Li, Y. Z. Deng, and J. K. Cheng, “Sensitive photothermal interferometric detection method for characterization of transparent plate samples,” Rev. Sci. Instrum. 67, 3649–3657 (1996).
  20. J. M. Harris and N. J. Dovichi, “Thermal lens calorimetry,” Anal. Chem. 52, 695A–706A (1980).
  21. A. E. Bruno, A. Paulus, and D. J. Bornhop, “Thermo-optical absorption detection in 25-μm-i.d. capillaries: capillary electrophoresis of Dansyl-amino acids mixtures,” Appl. Spectrosc. 45, 462–467 (1991).
  22. B. C. Li, Y. Z. Deng, and J. K. Cheng, “Pulsed photothermal phase-shift spectroscopy for weak absorption measurements,” Talanta 43, 627–633 (1996).

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